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Alternatively spliced isoforms of the human elk-1 mRNA within the 5′ UTR: implications for ELK-1 expression

The expression of cellular proteins that play central roles in the regulation of cell growth and differentiation is frequently tightly controlled at the level of translation initiation. In this article, we provide evidence that the ETS domain transcription factor ELK-1 forms part of this class of ge...

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Detalles Bibliográficos
Autores principales: Araud, Tanguy, Genolet, Raphael, Jaquier-Gubler, Pascale, Curran, Joseph
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1950554/
https://www.ncbi.nlm.nih.gov/pubmed/17591614
http://dx.doi.org/10.1093/nar/gkm482
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author Araud, Tanguy
Genolet, Raphael
Jaquier-Gubler, Pascale
Curran, Joseph
author_facet Araud, Tanguy
Genolet, Raphael
Jaquier-Gubler, Pascale
Curran, Joseph
author_sort Araud, Tanguy
collection PubMed
description The expression of cellular proteins that play central roles in the regulation of cell growth and differentiation is frequently tightly controlled at the level of translation initiation. In this article, we provide evidence that the ETS domain transcription factor ELK-1 forms part of this class of genes. Its mRNA 5′ UTR is composed of a complexed mosaic of elements, including uAUGs, uORFs and RNA structure, that interplay to modulate ribosomal access to the ELK-1 AUG start codon. Superimposed upon this is the generation of two different 5′ UTRs via alternative splicing. The two spliced isoforms show altered cellular and tissue distributions and behave differently in polysomal recruitment assays in the presence of the drug rapamycin. We propose that repression is therefore the sum of a series of interplaying negative elements within the 5′ UTRs, a situation which may reflect the need for tight translational control of ELK-1 in different tissues and under changing physiological conditions.
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spelling pubmed-19505542007-08-22 Alternatively spliced isoforms of the human elk-1 mRNA within the 5′ UTR: implications for ELK-1 expression Araud, Tanguy Genolet, Raphael Jaquier-Gubler, Pascale Curran, Joseph Nucleic Acids Res Molecular Biology The expression of cellular proteins that play central roles in the regulation of cell growth and differentiation is frequently tightly controlled at the level of translation initiation. In this article, we provide evidence that the ETS domain transcription factor ELK-1 forms part of this class of genes. Its mRNA 5′ UTR is composed of a complexed mosaic of elements, including uAUGs, uORFs and RNA structure, that interplay to modulate ribosomal access to the ELK-1 AUG start codon. Superimposed upon this is the generation of two different 5′ UTRs via alternative splicing. The two spliced isoforms show altered cellular and tissue distributions and behave differently in polysomal recruitment assays in the presence of the drug rapamycin. We propose that repression is therefore the sum of a series of interplaying negative elements within the 5′ UTRs, a situation which may reflect the need for tight translational control of ELK-1 in different tissues and under changing physiological conditions. Oxford University Press 2007-07 2007-06-25 /pmc/articles/PMC1950554/ /pubmed/17591614 http://dx.doi.org/10.1093/nar/gkm482 Text en © 2007 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Molecular Biology
Araud, Tanguy
Genolet, Raphael
Jaquier-Gubler, Pascale
Curran, Joseph
Alternatively spliced isoforms of the human elk-1 mRNA within the 5′ UTR: implications for ELK-1 expression
title Alternatively spliced isoforms of the human elk-1 mRNA within the 5′ UTR: implications for ELK-1 expression
title_full Alternatively spliced isoforms of the human elk-1 mRNA within the 5′ UTR: implications for ELK-1 expression
title_fullStr Alternatively spliced isoforms of the human elk-1 mRNA within the 5′ UTR: implications for ELK-1 expression
title_full_unstemmed Alternatively spliced isoforms of the human elk-1 mRNA within the 5′ UTR: implications for ELK-1 expression
title_short Alternatively spliced isoforms of the human elk-1 mRNA within the 5′ UTR: implications for ELK-1 expression
title_sort alternatively spliced isoforms of the human elk-1 mrna within the 5′ utr: implications for elk-1 expression
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1950554/
https://www.ncbi.nlm.nih.gov/pubmed/17591614
http://dx.doi.org/10.1093/nar/gkm482
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