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Comparative proteomics reveal characteristics of life-history transitions in a social insect

BACKGROUND: Honey bee (Apis mellifera) workers are characterized by complex social behavior. Their life-history is dominated by a period of within-nest activity followed by a phase of long-distance flights and foraging. General insights into insect metabolism imply that foraging onset is associated...

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Detalles Bibliográficos
Autores principales: Wolschin, Florian, Amdam, Gro V
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1964756/
https://www.ncbi.nlm.nih.gov/pubmed/17634121
http://dx.doi.org/10.1186/1477-5956-5-10
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author Wolschin, Florian
Amdam, Gro V
author_facet Wolschin, Florian
Amdam, Gro V
author_sort Wolschin, Florian
collection PubMed
description BACKGROUND: Honey bee (Apis mellifera) workers are characterized by complex social behavior. Their life-history is dominated by a period of within-nest activity followed by a phase of long-distance flights and foraging. General insights into insect metabolism imply that foraging onset is associated with fundamental metabolic changes, and theory on social evolution suggests metabolic adaptations that are advantageous for the colony as a whole. RESULTS: Here we address the life-history characteristics of workers with LC-MS/MS based relative quantification of major proteins. Our approach includes: i. Calculation of a false positive rate for the identifications, ii. Support of relative protein quantification results obtained from spectral count by non-parametric statistics, and iii. Correction for Type 1 error inflation using a bootstrap iteration analysis. Our data are consistent with the use of glucose as the main fuel for honey bee flight. Moreover, the data delivers information on the expression of ATPsynthases/ATPases, and provide new insights into nurse- and forager-specific patterns of protection against oxidative stress. CONCLUSION: The results show the suitability of this approach to investigate fundamental biochemical changes in an insect, and provide new evidence for metabolic specializations that occur during the social ontogeny of worker honey bees.
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spelling pubmed-19647562007-09-05 Comparative proteomics reveal characteristics of life-history transitions in a social insect Wolschin, Florian Amdam, Gro V Proteome Sci Research BACKGROUND: Honey bee (Apis mellifera) workers are characterized by complex social behavior. Their life-history is dominated by a period of within-nest activity followed by a phase of long-distance flights and foraging. General insights into insect metabolism imply that foraging onset is associated with fundamental metabolic changes, and theory on social evolution suggests metabolic adaptations that are advantageous for the colony as a whole. RESULTS: Here we address the life-history characteristics of workers with LC-MS/MS based relative quantification of major proteins. Our approach includes: i. Calculation of a false positive rate for the identifications, ii. Support of relative protein quantification results obtained from spectral count by non-parametric statistics, and iii. Correction for Type 1 error inflation using a bootstrap iteration analysis. Our data are consistent with the use of glucose as the main fuel for honey bee flight. Moreover, the data delivers information on the expression of ATPsynthases/ATPases, and provide new insights into nurse- and forager-specific patterns of protection against oxidative stress. CONCLUSION: The results show the suitability of this approach to investigate fundamental biochemical changes in an insect, and provide new evidence for metabolic specializations that occur during the social ontogeny of worker honey bees. BioMed Central 2007-07-17 /pmc/articles/PMC1964756/ /pubmed/17634121 http://dx.doi.org/10.1186/1477-5956-5-10 Text en Copyright © 2007 Wolschin and Amdam; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Wolschin, Florian
Amdam, Gro V
Comparative proteomics reveal characteristics of life-history transitions in a social insect
title Comparative proteomics reveal characteristics of life-history transitions in a social insect
title_full Comparative proteomics reveal characteristics of life-history transitions in a social insect
title_fullStr Comparative proteomics reveal characteristics of life-history transitions in a social insect
title_full_unstemmed Comparative proteomics reveal characteristics of life-history transitions in a social insect
title_short Comparative proteomics reveal characteristics of life-history transitions in a social insect
title_sort comparative proteomics reveal characteristics of life-history transitions in a social insect
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1964756/
https://www.ncbi.nlm.nih.gov/pubmed/17634121
http://dx.doi.org/10.1186/1477-5956-5-10
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