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Comparative proteomics reveal characteristics of life-history transitions in a social insect
BACKGROUND: Honey bee (Apis mellifera) workers are characterized by complex social behavior. Their life-history is dominated by a period of within-nest activity followed by a phase of long-distance flights and foraging. General insights into insect metabolism imply that foraging onset is associated...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1964756/ https://www.ncbi.nlm.nih.gov/pubmed/17634121 http://dx.doi.org/10.1186/1477-5956-5-10 |
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author | Wolschin, Florian Amdam, Gro V |
author_facet | Wolschin, Florian Amdam, Gro V |
author_sort | Wolschin, Florian |
collection | PubMed |
description | BACKGROUND: Honey bee (Apis mellifera) workers are characterized by complex social behavior. Their life-history is dominated by a period of within-nest activity followed by a phase of long-distance flights and foraging. General insights into insect metabolism imply that foraging onset is associated with fundamental metabolic changes, and theory on social evolution suggests metabolic adaptations that are advantageous for the colony as a whole. RESULTS: Here we address the life-history characteristics of workers with LC-MS/MS based relative quantification of major proteins. Our approach includes: i. Calculation of a false positive rate for the identifications, ii. Support of relative protein quantification results obtained from spectral count by non-parametric statistics, and iii. Correction for Type 1 error inflation using a bootstrap iteration analysis. Our data are consistent with the use of glucose as the main fuel for honey bee flight. Moreover, the data delivers information on the expression of ATPsynthases/ATPases, and provide new insights into nurse- and forager-specific patterns of protection against oxidative stress. CONCLUSION: The results show the suitability of this approach to investigate fundamental biochemical changes in an insect, and provide new evidence for metabolic specializations that occur during the social ontogeny of worker honey bees. |
format | Text |
id | pubmed-1964756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-19647562007-09-05 Comparative proteomics reveal characteristics of life-history transitions in a social insect Wolschin, Florian Amdam, Gro V Proteome Sci Research BACKGROUND: Honey bee (Apis mellifera) workers are characterized by complex social behavior. Their life-history is dominated by a period of within-nest activity followed by a phase of long-distance flights and foraging. General insights into insect metabolism imply that foraging onset is associated with fundamental metabolic changes, and theory on social evolution suggests metabolic adaptations that are advantageous for the colony as a whole. RESULTS: Here we address the life-history characteristics of workers with LC-MS/MS based relative quantification of major proteins. Our approach includes: i. Calculation of a false positive rate for the identifications, ii. Support of relative protein quantification results obtained from spectral count by non-parametric statistics, and iii. Correction for Type 1 error inflation using a bootstrap iteration analysis. Our data are consistent with the use of glucose as the main fuel for honey bee flight. Moreover, the data delivers information on the expression of ATPsynthases/ATPases, and provide new insights into nurse- and forager-specific patterns of protection against oxidative stress. CONCLUSION: The results show the suitability of this approach to investigate fundamental biochemical changes in an insect, and provide new evidence for metabolic specializations that occur during the social ontogeny of worker honey bees. BioMed Central 2007-07-17 /pmc/articles/PMC1964756/ /pubmed/17634121 http://dx.doi.org/10.1186/1477-5956-5-10 Text en Copyright © 2007 Wolschin and Amdam; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Wolschin, Florian Amdam, Gro V Comparative proteomics reveal characteristics of life-history transitions in a social insect |
title | Comparative proteomics reveal characteristics of life-history transitions in a social insect |
title_full | Comparative proteomics reveal characteristics of life-history transitions in a social insect |
title_fullStr | Comparative proteomics reveal characteristics of life-history transitions in a social insect |
title_full_unstemmed | Comparative proteomics reveal characteristics of life-history transitions in a social insect |
title_short | Comparative proteomics reveal characteristics of life-history transitions in a social insect |
title_sort | comparative proteomics reveal characteristics of life-history transitions in a social insect |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1964756/ https://www.ncbi.nlm.nih.gov/pubmed/17634121 http://dx.doi.org/10.1186/1477-5956-5-10 |
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