Micronucleus formation in human tumour cells: lack of correlation with radiosensitivity.

The micronucleus (MN) test has been carefully characterized in four human tumour cell lines of widely differing radiosensitivity. Two radioresistant bladder carcinoma cell lines (MGH-U1 and RT112), one sensitive medulloblastoma cell line (D283MED) and a sensitive neuroblastoma cell line (HX142) were...

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Detalles Bibliográficos
Autores principales: Bush, C., McMillan, T. J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1993
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968207/
https://www.ncbi.nlm.nih.gov/pubmed/8427770
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author Bush, C.
McMillan, T. J.
author_facet Bush, C.
McMillan, T. J.
author_sort Bush, C.
collection PubMed
description The micronucleus (MN) test has been carefully characterized in four human tumour cell lines of widely differing radiosensitivity. Two radioresistant bladder carcinoma cell lines (MGH-U1 and RT112), one sensitive medulloblastoma cell line (D283MED) and a sensitive neuroblastoma cell line (HX142) were used. The number of MN per Gy of ionising radiation was 0.13 for HX142, 0.17 for D283MED, 0.21 for RT112 and 0.26 for MGH-U1. This does not rank the cell lines in the same order of radiosensitivity as clonogenic cell survival where the surviving fraction at 2 Gy (SF2) was 0.11 for HX142, 0.2 for D283MED, 0.62 for RT112 and 0.53 for MGH-U1. This discrepancy between MN formation and cell death leaves doubt as to the potential usefulness of the MN test as a rapid assay of radiosensitivity but it has potential implications for the mechanistic basis of radiosensitivity in these cells.
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spelling pubmed-19682072009-09-10 Micronucleus formation in human tumour cells: lack of correlation with radiosensitivity. Bush, C. McMillan, T. J. Br J Cancer Research Article The micronucleus (MN) test has been carefully characterized in four human tumour cell lines of widely differing radiosensitivity. Two radioresistant bladder carcinoma cell lines (MGH-U1 and RT112), one sensitive medulloblastoma cell line (D283MED) and a sensitive neuroblastoma cell line (HX142) were used. The number of MN per Gy of ionising radiation was 0.13 for HX142, 0.17 for D283MED, 0.21 for RT112 and 0.26 for MGH-U1. This does not rank the cell lines in the same order of radiosensitivity as clonogenic cell survival where the surviving fraction at 2 Gy (SF2) was 0.11 for HX142, 0.2 for D283MED, 0.62 for RT112 and 0.53 for MGH-U1. This discrepancy between MN formation and cell death leaves doubt as to the potential usefulness of the MN test as a rapid assay of radiosensitivity but it has potential implications for the mechanistic basis of radiosensitivity in these cells. Nature Publishing Group 1993-01 /pmc/articles/PMC1968207/ /pubmed/8427770 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Bush, C.
McMillan, T. J.
Micronucleus formation in human tumour cells: lack of correlation with radiosensitivity.
title Micronucleus formation in human tumour cells: lack of correlation with radiosensitivity.
title_full Micronucleus formation in human tumour cells: lack of correlation with radiosensitivity.
title_fullStr Micronucleus formation in human tumour cells: lack of correlation with radiosensitivity.
title_full_unstemmed Micronucleus formation in human tumour cells: lack of correlation with radiosensitivity.
title_short Micronucleus formation in human tumour cells: lack of correlation with radiosensitivity.
title_sort micronucleus formation in human tumour cells: lack of correlation with radiosensitivity.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968207/
https://www.ncbi.nlm.nih.gov/pubmed/8427770
work_keys_str_mv AT bushc micronucleusformationinhumantumourcellslackofcorrelationwithradiosensitivity
AT mcmillantj micronucleusformationinhumantumourcellslackofcorrelationwithradiosensitivity

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