Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.

A cell line (SMKT-R3) established from human renal cell carcinoma was characterized for the presence of sulfolipids and glycolipid sulfotransferases. Sulfolipids were found to constitute a large part of the acidic glycolipid fraction in SMKT-R3 cells. These findings were confirmed by metabolic label...

Descripción completa

Detalles Bibliográficos
Autores principales: Kobayashi, T., Honke, K., Kamio, K., Sakakibara, N., Gasa, S., Miyao, N., Tsukamoto, T., Ishizuka, I., Miyazaki, T., Makita, A.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1993
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968242/
https://www.ncbi.nlm.nih.gov/pubmed/8094007
_version_ 1782134698111139840
author Kobayashi, T.
Honke, K.
Kamio, K.
Sakakibara, N.
Gasa, S.
Miyao, N.
Tsukamoto, T.
Ishizuka, I.
Miyazaki, T.
Makita, A.
author_facet Kobayashi, T.
Honke, K.
Kamio, K.
Sakakibara, N.
Gasa, S.
Miyao, N.
Tsukamoto, T.
Ishizuka, I.
Miyazaki, T.
Makita, A.
author_sort Kobayashi, T.
collection PubMed
description A cell line (SMKT-R3) established from human renal cell carcinoma was characterized for the presence of sulfolipids and glycolipid sulfotransferases. Sulfolipids were found to constitute a large part of the acidic glycolipid fraction in SMKT-R3 cells. These findings were confirmed by metabolic labelling with 35S-sulfate. These sulfolipids were expressed at the surface of SMKT-R3 cells as ascertained by cytofluorometry using a monoclonal antibody directed to sulfolipids. Furthermore, markedly high activity levels of glycolipid sulfotransferases were observed in SMKT-R3 cells compared with other cell lines. These results suggest that the increased synthesis of sulfolipids in renal cell carcinoma tissue (Sakakibara et al., 1989. Cancer Res., 49, 335-339) is due to the elevation of the sulfotransferase activities of renal carcinoma cells themselves. IMAGES:
format Text
id pubmed-1968242
institution National Center for Biotechnology Information
language English
publishDate 1993
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-19682422009-09-10 Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells. Kobayashi, T. Honke, K. Kamio, K. Sakakibara, N. Gasa, S. Miyao, N. Tsukamoto, T. Ishizuka, I. Miyazaki, T. Makita, A. Br J Cancer Research Article A cell line (SMKT-R3) established from human renal cell carcinoma was characterized for the presence of sulfolipids and glycolipid sulfotransferases. Sulfolipids were found to constitute a large part of the acidic glycolipid fraction in SMKT-R3 cells. These findings were confirmed by metabolic labelling with 35S-sulfate. These sulfolipids were expressed at the surface of SMKT-R3 cells as ascertained by cytofluorometry using a monoclonal antibody directed to sulfolipids. Furthermore, markedly high activity levels of glycolipid sulfotransferases were observed in SMKT-R3 cells compared with other cell lines. These results suggest that the increased synthesis of sulfolipids in renal cell carcinoma tissue (Sakakibara et al., 1989. Cancer Res., 49, 335-339) is due to the elevation of the sulfotransferase activities of renal carcinoma cells themselves. IMAGES: Nature Publishing Group 1993-01 /pmc/articles/PMC1968242/ /pubmed/8094007 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Kobayashi, T.
Honke, K.
Kamio, K.
Sakakibara, N.
Gasa, S.
Miyao, N.
Tsukamoto, T.
Ishizuka, I.
Miyazaki, T.
Makita, A.
Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.
title Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.
title_full Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.
title_fullStr Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.
title_full_unstemmed Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.
title_short Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.
title_sort sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968242/
https://www.ncbi.nlm.nih.gov/pubmed/8094007
work_keys_str_mv AT kobayashit sulfolipidsandglycolipidsulfotransferaseactivitiesinhumanrenalcellcarcinomacells
AT honkek sulfolipidsandglycolipidsulfotransferaseactivitiesinhumanrenalcellcarcinomacells
AT kamiok sulfolipidsandglycolipidsulfotransferaseactivitiesinhumanrenalcellcarcinomacells
AT sakakibaran sulfolipidsandglycolipidsulfotransferaseactivitiesinhumanrenalcellcarcinomacells
AT gasas sulfolipidsandglycolipidsulfotransferaseactivitiesinhumanrenalcellcarcinomacells
AT miyaon sulfolipidsandglycolipidsulfotransferaseactivitiesinhumanrenalcellcarcinomacells
AT tsukamotot sulfolipidsandglycolipidsulfotransferaseactivitiesinhumanrenalcellcarcinomacells
AT ishizukai sulfolipidsandglycolipidsulfotransferaseactivitiesinhumanrenalcellcarcinomacells
AT miyazakit sulfolipidsandglycolipidsulfotransferaseactivitiesinhumanrenalcellcarcinomacells
AT makitaa sulfolipidsandglycolipidsulfotransferaseactivitiesinhumanrenalcellcarcinomacells

Ejemplares similares