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Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells.

There is considerable interest in immunohistochemical markers of proliferation which are suitable for use on routinely fixed clinical material. The novel proliferation-associated antibody Ki-S1 shows promise in this respect. In this study we have: (i) defined the pattern of Ki-S1 labelling relative...

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Autores principales: Camplejohn, R. S., Brock, A., Barnes, D. M., Gillett, C., Raikundalia, B., Kreipe, H., Parwaresch, M. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968331/
https://www.ncbi.nlm.nih.gov/pubmed/7682430
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author Camplejohn, R. S.
Brock, A.
Barnes, D. M.
Gillett, C.
Raikundalia, B.
Kreipe, H.
Parwaresch, M. R.
author_facet Camplejohn, R. S.
Brock, A.
Barnes, D. M.
Gillett, C.
Raikundalia, B.
Kreipe, H.
Parwaresch, M. R.
author_sort Camplejohn, R. S.
collection PubMed
description There is considerable interest in immunohistochemical markers of proliferation which are suitable for use on routinely fixed clinical material. The novel proliferation-associated antibody Ki-S1 shows promise in this respect. In this study we have: (i) defined the pattern of Ki-S1 labelling relative to the cell cycle phase; (ii) investigated the labelling pattern with Ki-S1 on a human breast cell line (ZR75) under varying proliferative conditions induced by serum deprivation and refeeding; (iii) examined in a flow cytometric study Ki-S1 staining in archival, clinical breast carcinoma samples. In exponentially growing cells Ki-S1 showed a marked cell cycle phase-specific variation in staining intensity which increased linearly through the S-phase, was high in G2 and reached its peak in mitosis. Ki-S1 staining intensity mirrored the changes in proliferative activity of ZR75 cells during serum deprivation and refeeding. In a small series of human breast carcinoma, Ki-S1 staining intensity correlated with S-phase fraction (SPF) derived from DNA profiles. The antigen labelled by Ki-S1 is extremely robust, resisting degradation by fixation and by an aggressive enzymic tissue disaggregation method. Ki-S1 warrants further investigation as a proliferation-related marker, particularly for routine clinical application.
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spelling pubmed-19683312009-09-10 Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells. Camplejohn, R. S. Brock, A. Barnes, D. M. Gillett, C. Raikundalia, B. Kreipe, H. Parwaresch, M. R. Br J Cancer Research Article There is considerable interest in immunohistochemical markers of proliferation which are suitable for use on routinely fixed clinical material. The novel proliferation-associated antibody Ki-S1 shows promise in this respect. In this study we have: (i) defined the pattern of Ki-S1 labelling relative to the cell cycle phase; (ii) investigated the labelling pattern with Ki-S1 on a human breast cell line (ZR75) under varying proliferative conditions induced by serum deprivation and refeeding; (iii) examined in a flow cytometric study Ki-S1 staining in archival, clinical breast carcinoma samples. In exponentially growing cells Ki-S1 showed a marked cell cycle phase-specific variation in staining intensity which increased linearly through the S-phase, was high in G2 and reached its peak in mitosis. Ki-S1 staining intensity mirrored the changes in proliferative activity of ZR75 cells during serum deprivation and refeeding. In a small series of human breast carcinoma, Ki-S1 staining intensity correlated with S-phase fraction (SPF) derived from DNA profiles. The antigen labelled by Ki-S1 is extremely robust, resisting degradation by fixation and by an aggressive enzymic tissue disaggregation method. Ki-S1 warrants further investigation as a proliferation-related marker, particularly for routine clinical application. Nature Publishing Group 1993-04 /pmc/articles/PMC1968331/ /pubmed/7682430 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Camplejohn, R. S.
Brock, A.
Barnes, D. M.
Gillett, C.
Raikundalia, B.
Kreipe, H.
Parwaresch, M. R.
Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells.
title Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells.
title_full Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells.
title_fullStr Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells.
title_full_unstemmed Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells.
title_short Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells.
title_sort ki-s1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968331/
https://www.ncbi.nlm.nih.gov/pubmed/7682430
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