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Prognostic significance of TP53 alterations in breast carcinoma.
Constant denaturant gel electrophoresis (CDGE) was used to screen 179 breast carcinomas for mutations in the conserved regions of the TP53 gene (exons 5 through 8). Mutations were found in 35 of 163 primary tumours (21%) and in 5 of 16 metastases (31%) and resided predominantly in exon 7. The majori...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1993
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968415/ https://www.ncbi.nlm.nih.gov/pubmed/8102535 |
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author | Andersen, T. I. Holm, R. Nesland, J. M. Heimdal, K. R. Ottestad, L. Børresen, A. L. |
author_facet | Andersen, T. I. Holm, R. Nesland, J. M. Heimdal, K. R. Ottestad, L. Børresen, A. L. |
author_sort | Andersen, T. I. |
collection | PubMed |
description | Constant denaturant gel electrophoresis (CDGE) was used to screen 179 breast carcinomas for mutations in the conserved regions of the TP53 gene (exons 5 through 8). Mutations were found in 35 of 163 primary tumours (21%) and in 5 of 16 metastases (31%) and resided predominantly in exon 7. The majority of the mutations were G:C-->A:T transitions. Immunohistochemistry demonstrated nuclear accumulation of p53 protein in 35 of 162 primary tumours (22%) and in four of 15 metastases (27%). TP53 mutation was strongly associated with nuclear accumulation of p53 protein. In total 42 of 163 primary tumours (26%) and 5 of 16 metastases (31%) were demonstrated to contain TP53 alterations (mutation and/or nuclear protein accumulation). TP53 alteration in primary tumour was significantly associated with the following parameters: positive node status, T status > 1, negative oestrogen receptor status, negative progesterone receptor status, presence of ERBB2 gene amplification, and invasive ductal histology. Furthermore, there were statistically significant associations, independent of other prognostic factors, between TP53 alterations in primary tumour and disease-free and overall survival. IMAGES: |
format | Text |
id | pubmed-1968415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1993 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-19684152009-09-10 Prognostic significance of TP53 alterations in breast carcinoma. Andersen, T. I. Holm, R. Nesland, J. M. Heimdal, K. R. Ottestad, L. Børresen, A. L. Br J Cancer Research Article Constant denaturant gel electrophoresis (CDGE) was used to screen 179 breast carcinomas for mutations in the conserved regions of the TP53 gene (exons 5 through 8). Mutations were found in 35 of 163 primary tumours (21%) and in 5 of 16 metastases (31%) and resided predominantly in exon 7. The majority of the mutations were G:C-->A:T transitions. Immunohistochemistry demonstrated nuclear accumulation of p53 protein in 35 of 162 primary tumours (22%) and in four of 15 metastases (27%). TP53 mutation was strongly associated with nuclear accumulation of p53 protein. In total 42 of 163 primary tumours (26%) and 5 of 16 metastases (31%) were demonstrated to contain TP53 alterations (mutation and/or nuclear protein accumulation). TP53 alteration in primary tumour was significantly associated with the following parameters: positive node status, T status > 1, negative oestrogen receptor status, negative progesterone receptor status, presence of ERBB2 gene amplification, and invasive ductal histology. Furthermore, there were statistically significant associations, independent of other prognostic factors, between TP53 alterations in primary tumour and disease-free and overall survival. IMAGES: Nature Publishing Group 1993-09 /pmc/articles/PMC1968415/ /pubmed/8102535 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Andersen, T. I. Holm, R. Nesland, J. M. Heimdal, K. R. Ottestad, L. Børresen, A. L. Prognostic significance of TP53 alterations in breast carcinoma. |
title | Prognostic significance of TP53 alterations in breast carcinoma. |
title_full | Prognostic significance of TP53 alterations in breast carcinoma. |
title_fullStr | Prognostic significance of TP53 alterations in breast carcinoma. |
title_full_unstemmed | Prognostic significance of TP53 alterations in breast carcinoma. |
title_short | Prognostic significance of TP53 alterations in breast carcinoma. |
title_sort | prognostic significance of tp53 alterations in breast carcinoma. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968415/ https://www.ncbi.nlm.nih.gov/pubmed/8102535 |
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