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Fibroblast stimulation of breast cancer cell growth in a serum-free system.

Conditioned media from 14 short term fibroblast cell lines were mitogenic for human breast cancer cells with different steroid receptor profiles in serum-free culture. Fibroblast-conditioned medium stimulated tritiated thymidine incorporation in short term culture and growth in a longer proliferatio...

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Detalles Bibliográficos
Autores principales: Ryan, M. C., Orr, D. J., Horgan, K.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968494/
https://www.ncbi.nlm.nih.gov/pubmed/8512812
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author Ryan, M. C.
Orr, D. J.
Horgan, K.
author_facet Ryan, M. C.
Orr, D. J.
Horgan, K.
author_sort Ryan, M. C.
collection PubMed
description Conditioned media from 14 short term fibroblast cell lines were mitogenic for human breast cancer cells with different steroid receptor profiles in serum-free culture. Fibroblast-conditioned medium stimulated tritiated thymidine incorporation in short term culture and growth in a longer proliferation study as measured by the MTT colorimetric assay. Conditioned media from benign and malignant epithelial cells were non-stimulatory for breast cancer cells but that derived from endothelial cells showed similar stimulation to fibroblasts. Partial purification of fibroblast-conditioned medium identified a peptide with a molecular weight of approximately 8 kDa that showed no affinity for heparin and was mitogenic for MCF-7 breast cancer cells.
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spelling pubmed-19684942009-09-10 Fibroblast stimulation of breast cancer cell growth in a serum-free system. Ryan, M. C. Orr, D. J. Horgan, K. Br J Cancer Research Article Conditioned media from 14 short term fibroblast cell lines were mitogenic for human breast cancer cells with different steroid receptor profiles in serum-free culture. Fibroblast-conditioned medium stimulated tritiated thymidine incorporation in short term culture and growth in a longer proliferation study as measured by the MTT colorimetric assay. Conditioned media from benign and malignant epithelial cells were non-stimulatory for breast cancer cells but that derived from endothelial cells showed similar stimulation to fibroblasts. Partial purification of fibroblast-conditioned medium identified a peptide with a molecular weight of approximately 8 kDa that showed no affinity for heparin and was mitogenic for MCF-7 breast cancer cells. Nature Publishing Group 1993-06 /pmc/articles/PMC1968494/ /pubmed/8512812 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Ryan, M. C.
Orr, D. J.
Horgan, K.
Fibroblast stimulation of breast cancer cell growth in a serum-free system.
title Fibroblast stimulation of breast cancer cell growth in a serum-free system.
title_full Fibroblast stimulation of breast cancer cell growth in a serum-free system.
title_fullStr Fibroblast stimulation of breast cancer cell growth in a serum-free system.
title_full_unstemmed Fibroblast stimulation of breast cancer cell growth in a serum-free system.
title_short Fibroblast stimulation of breast cancer cell growth in a serum-free system.
title_sort fibroblast stimulation of breast cancer cell growth in a serum-free system.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968494/
https://www.ncbi.nlm.nih.gov/pubmed/8512812
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