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Fibroblast stimulation of breast cancer cell growth in a serum-free system.
Conditioned media from 14 short term fibroblast cell lines were mitogenic for human breast cancer cells with different steroid receptor profiles in serum-free culture. Fibroblast-conditioned medium stimulated tritiated thymidine incorporation in short term culture and growth in a longer proliferatio...
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1993
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968494/ https://www.ncbi.nlm.nih.gov/pubmed/8512812 |
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author | Ryan, M. C. Orr, D. J. Horgan, K. |
author_facet | Ryan, M. C. Orr, D. J. Horgan, K. |
author_sort | Ryan, M. C. |
collection | PubMed |
description | Conditioned media from 14 short term fibroblast cell lines were mitogenic for human breast cancer cells with different steroid receptor profiles in serum-free culture. Fibroblast-conditioned medium stimulated tritiated thymidine incorporation in short term culture and growth in a longer proliferation study as measured by the MTT colorimetric assay. Conditioned media from benign and malignant epithelial cells were non-stimulatory for breast cancer cells but that derived from endothelial cells showed similar stimulation to fibroblasts. Partial purification of fibroblast-conditioned medium identified a peptide with a molecular weight of approximately 8 kDa that showed no affinity for heparin and was mitogenic for MCF-7 breast cancer cells. |
format | Text |
id | pubmed-1968494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1993 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-19684942009-09-10 Fibroblast stimulation of breast cancer cell growth in a serum-free system. Ryan, M. C. Orr, D. J. Horgan, K. Br J Cancer Research Article Conditioned media from 14 short term fibroblast cell lines were mitogenic for human breast cancer cells with different steroid receptor profiles in serum-free culture. Fibroblast-conditioned medium stimulated tritiated thymidine incorporation in short term culture and growth in a longer proliferation study as measured by the MTT colorimetric assay. Conditioned media from benign and malignant epithelial cells were non-stimulatory for breast cancer cells but that derived from endothelial cells showed similar stimulation to fibroblasts. Partial purification of fibroblast-conditioned medium identified a peptide with a molecular weight of approximately 8 kDa that showed no affinity for heparin and was mitogenic for MCF-7 breast cancer cells. Nature Publishing Group 1993-06 /pmc/articles/PMC1968494/ /pubmed/8512812 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Ryan, M. C. Orr, D. J. Horgan, K. Fibroblast stimulation of breast cancer cell growth in a serum-free system. |
title | Fibroblast stimulation of breast cancer cell growth in a serum-free system. |
title_full | Fibroblast stimulation of breast cancer cell growth in a serum-free system. |
title_fullStr | Fibroblast stimulation of breast cancer cell growth in a serum-free system. |
title_full_unstemmed | Fibroblast stimulation of breast cancer cell growth in a serum-free system. |
title_short | Fibroblast stimulation of breast cancer cell growth in a serum-free system. |
title_sort | fibroblast stimulation of breast cancer cell growth in a serum-free system. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968494/ https://www.ncbi.nlm.nih.gov/pubmed/8512812 |
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