Evidence for field change in oral cancer based on cytokeratin expression.

It was hypothesised that one may be able to visualise field changes, which are proposed to exist around tumours, as alterations in keratin intermediate filament protein expression. Standard immunohistochemical analysis using a panel of monoclonal anti-keratin antibodies was applied to fresh tissue s...

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Detalles Bibliográficos
Autores principales: Ogden, G. R., Lane, E. B., Hopwood, D. V., Chisholm, D. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1993
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968511/
https://www.ncbi.nlm.nih.gov/pubmed/7685618
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author Ogden, G. R.
Lane, E. B.
Hopwood, D. V.
Chisholm, D. M.
author_facet Ogden, G. R.
Lane, E. B.
Hopwood, D. V.
Chisholm, D. M.
author_sort Ogden, G. R.
collection PubMed
description It was hypothesised that one may be able to visualise field changes, which are proposed to exist around tumours, as alterations in keratin intermediate filament protein expression. Standard immunohistochemical analysis using a panel of monoclonal anti-keratin antibodies was applied to fresh tissue sections to look for subtle changes in epithelial differentiation not visible in H&E sections. Such changes were observed in clinically normal epithelium from oral cancer patients, involving primarily substantial expression of keratins K8/K7 (using CAM 5.2) in the basal cells of 12 out of 34 biopsies, and also a trend towards a reduction in the complexity of keratin differentiation. Monitoring such changes may prove to be a valuable adjunct to conventional H&E staining if found to have prognostic and diagnostic significance. IMAGES:
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spelling pubmed-19685112009-09-10 Evidence for field change in oral cancer based on cytokeratin expression. Ogden, G. R. Lane, E. B. Hopwood, D. V. Chisholm, D. M. Br J Cancer Research Article It was hypothesised that one may be able to visualise field changes, which are proposed to exist around tumours, as alterations in keratin intermediate filament protein expression. Standard immunohistochemical analysis using a panel of monoclonal anti-keratin antibodies was applied to fresh tissue sections to look for subtle changes in epithelial differentiation not visible in H&E sections. Such changes were observed in clinically normal epithelium from oral cancer patients, involving primarily substantial expression of keratins K8/K7 (using CAM 5.2) in the basal cells of 12 out of 34 biopsies, and also a trend towards a reduction in the complexity of keratin differentiation. Monitoring such changes may prove to be a valuable adjunct to conventional H&E staining if found to have prognostic and diagnostic significance. IMAGES: Nature Publishing Group 1993-06 /pmc/articles/PMC1968511/ /pubmed/7685618 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Ogden, G. R.
Lane, E. B.
Hopwood, D. V.
Chisholm, D. M.
Evidence for field change in oral cancer based on cytokeratin expression.
title Evidence for field change in oral cancer based on cytokeratin expression.
title_full Evidence for field change in oral cancer based on cytokeratin expression.
title_fullStr Evidence for field change in oral cancer based on cytokeratin expression.
title_full_unstemmed Evidence for field change in oral cancer based on cytokeratin expression.
title_short Evidence for field change in oral cancer based on cytokeratin expression.
title_sort evidence for field change in oral cancer based on cytokeratin expression.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968511/
https://www.ncbi.nlm.nih.gov/pubmed/7685618
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