Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique.

We studied 51 paired samples of tissue sections and cytosol extracts from patients with breast cancer. A very high affinity monoclonal antibody to human p53 protein, DO-1, and polyclonal serum CM-1 to p53 protein were used for two site ELISA assays and CM-1 was used for immunohistochemistry to detec...

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Detalles Bibliográficos
Autores principales: Vojtĕsek, B., Fisher, C. J., Barnes, D. M., Lane, D. P.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1993
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968521/
https://www.ncbi.nlm.nih.gov/pubmed/7685617
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author Vojtĕsek, B.
Fisher, C. J.
Barnes, D. M.
Lane, D. P.
author_facet Vojtĕsek, B.
Fisher, C. J.
Barnes, D. M.
Lane, D. P.
author_sort Vojtĕsek, B.
collection PubMed
description We studied 51 paired samples of tissue sections and cytosol extracts from patients with breast cancer. A very high affinity monoclonal antibody to human p53 protein, DO-1, and polyclonal serum CM-1 to p53 protein were used for two site ELISA assays and CM-1 was used for immunohistochemistry to detect p53 protein accumulation in breast cancer samples. Eighteen carcinomas were positive for p53 by tissue staining and ELISA assay. Nineteen tumours were negative by ELISA and immunohistochemistry, and 14 cases with low levels of positive staining by immunohistochemistry were negative by the ELISA assay. A statistically significant correlation has been found between the degree of staining and the amount of p53 protein measured by ELISA (Pearson's correlation coefficient r = 0.59, P < 0.00001). Our ELISA assay offers an alternative approach to evaluating the p53 status of breast biopsy material, using cytosol extracts routinely prepared for steroid hormone receptor assays. This assay should also be of general application to other situations where the level of p53 protein needs to be determined. IMAGES:
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spelling pubmed-19685212009-09-10 Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique. Vojtĕsek, B. Fisher, C. J. Barnes, D. M. Lane, D. P. Br J Cancer Research Article We studied 51 paired samples of tissue sections and cytosol extracts from patients with breast cancer. A very high affinity monoclonal antibody to human p53 protein, DO-1, and polyclonal serum CM-1 to p53 protein were used for two site ELISA assays and CM-1 was used for immunohistochemistry to detect p53 protein accumulation in breast cancer samples. Eighteen carcinomas were positive for p53 by tissue staining and ELISA assay. Nineteen tumours were negative by ELISA and immunohistochemistry, and 14 cases with low levels of positive staining by immunohistochemistry were negative by the ELISA assay. A statistically significant correlation has been found between the degree of staining and the amount of p53 protein measured by ELISA (Pearson's correlation coefficient r = 0.59, P < 0.00001). Our ELISA assay offers an alternative approach to evaluating the p53 status of breast biopsy material, using cytosol extracts routinely prepared for steroid hormone receptor assays. This assay should also be of general application to other situations where the level of p53 protein needs to be determined. IMAGES: Nature Publishing Group 1993-06 /pmc/articles/PMC1968521/ /pubmed/7685617 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Vojtĕsek, B.
Fisher, C. J.
Barnes, D. M.
Lane, D. P.
Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique.
title Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique.
title_full Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique.
title_fullStr Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique.
title_full_unstemmed Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique.
title_short Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique.
title_sort comparison between p53 staining in tissue sections and p53 proteins levels measured by an elisa technique.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968521/
https://www.ncbi.nlm.nih.gov/pubmed/7685617
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