Expression of topoisomerase II alpha and beta in an adenocarcinoma cell line carrying amplified topoisomerase II alpha and retinoic acid receptor alpha genes.

Human topoisomerase II enzymes are targets for a number of widely used anticancer agents. We have analysed a lung adenocarcinoma cell line CALU3, which has co-amplified topoisomerase II alpha and ERBB2 sequences, for the structure of the amplicon and for expression of both topoisomerase II alpha and...

Descripción completa

Detalles Bibliográficos
Autores principales: Coutts, J., Plumb, J. A., Brown, R., Keith, W. N.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1993
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968606/
https://www.ncbi.nlm.nih.gov/pubmed/8398710
_version_ 1782134777342590976
author Coutts, J.
Plumb, J. A.
Brown, R.
Keith, W. N.
author_facet Coutts, J.
Plumb, J. A.
Brown, R.
Keith, W. N.
author_sort Coutts, J.
collection PubMed
description Human topoisomerase II enzymes are targets for a number of widely used anticancer agents. We have analysed a lung adenocarcinoma cell line CALU3, which has co-amplified topoisomerase II alpha and ERBB2 sequences, for the structure of the amplicon and for expression of both topoisomerase II alpha and beta. The region of chromosome 17q amplified in CALU3 also includes the retinoic acid receptor alpha locus and is therefore similar to the amplicon observed in breast cancers carrying amplified topoisomerase II alpha and retinoic acid receptor sequences. The use of fluorescence in situ hybridisation localises the amplified topoisomerase II alpha sequences to a cluster on one chromosome with single copies localised to others. CALU3 express high levels of topoisomerase II alpha is determined by Western blot, immunofluorescence and enzyme activity. The enzyme activity extracted from CALU3 is sensitive to inhibition by the topoisomerase II poison etoposide. Topoisomerase II beta expression was observed in three lung cancer cell lines including CALU3 and was confined to the nucleoli. Thus, the CALU3 cell line is an ideal model to study the amplification and expression of topoisomerase II alpha in adenocarcinomas. IMAGES:
format Text
id pubmed-1968606
institution National Center for Biotechnology Information
language English
publishDate 1993
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-19686062009-09-10 Expression of topoisomerase II alpha and beta in an adenocarcinoma cell line carrying amplified topoisomerase II alpha and retinoic acid receptor alpha genes. Coutts, J. Plumb, J. A. Brown, R. Keith, W. N. Br J Cancer Research Article Human topoisomerase II enzymes are targets for a number of widely used anticancer agents. We have analysed a lung adenocarcinoma cell line CALU3, which has co-amplified topoisomerase II alpha and ERBB2 sequences, for the structure of the amplicon and for expression of both topoisomerase II alpha and beta. The region of chromosome 17q amplified in CALU3 also includes the retinoic acid receptor alpha locus and is therefore similar to the amplicon observed in breast cancers carrying amplified topoisomerase II alpha and retinoic acid receptor sequences. The use of fluorescence in situ hybridisation localises the amplified topoisomerase II alpha sequences to a cluster on one chromosome with single copies localised to others. CALU3 express high levels of topoisomerase II alpha is determined by Western blot, immunofluorescence and enzyme activity. The enzyme activity extracted from CALU3 is sensitive to inhibition by the topoisomerase II poison etoposide. Topoisomerase II beta expression was observed in three lung cancer cell lines including CALU3 and was confined to the nucleoli. Thus, the CALU3 cell line is an ideal model to study the amplification and expression of topoisomerase II alpha in adenocarcinomas. IMAGES: Nature Publishing Group 1993-10 /pmc/articles/PMC1968606/ /pubmed/8398710 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Coutts, J.
Plumb, J. A.
Brown, R.
Keith, W. N.
Expression of topoisomerase II alpha and beta in an adenocarcinoma cell line carrying amplified topoisomerase II alpha and retinoic acid receptor alpha genes.
title Expression of topoisomerase II alpha and beta in an adenocarcinoma cell line carrying amplified topoisomerase II alpha and retinoic acid receptor alpha genes.
title_full Expression of topoisomerase II alpha and beta in an adenocarcinoma cell line carrying amplified topoisomerase II alpha and retinoic acid receptor alpha genes.
title_fullStr Expression of topoisomerase II alpha and beta in an adenocarcinoma cell line carrying amplified topoisomerase II alpha and retinoic acid receptor alpha genes.
title_full_unstemmed Expression of topoisomerase II alpha and beta in an adenocarcinoma cell line carrying amplified topoisomerase II alpha and retinoic acid receptor alpha genes.
title_short Expression of topoisomerase II alpha and beta in an adenocarcinoma cell line carrying amplified topoisomerase II alpha and retinoic acid receptor alpha genes.
title_sort expression of topoisomerase ii alpha and beta in an adenocarcinoma cell line carrying amplified topoisomerase ii alpha and retinoic acid receptor alpha genes.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968606/
https://www.ncbi.nlm.nih.gov/pubmed/8398710
work_keys_str_mv AT couttsj expressionoftopoisomeraseiialphaandbetainanadenocarcinomacelllinecarryingamplifiedtopoisomeraseiialphaandretinoicacidreceptoralphagenes
AT plumbja expressionoftopoisomeraseiialphaandbetainanadenocarcinomacelllinecarryingamplifiedtopoisomeraseiialphaandretinoicacidreceptoralphagenes
AT brownr expressionoftopoisomeraseiialphaandbetainanadenocarcinomacelllinecarryingamplifiedtopoisomeraseiialphaandretinoicacidreceptoralphagenes
AT keithwn expressionoftopoisomeraseiialphaandbetainanadenocarcinomacelllinecarryingamplifiedtopoisomeraseiialphaandretinoicacidreceptoralphagenes

Ejemplares similares