Malignant progression of SV40-immortalised human milk epithelial cells.

A human breast epithelial cell line (Hu-MI), established by microinjecting SV40 DNA into human milk epithelial cells, exhibits the phenotype of luminal epithelial cells and is neither clonogenic nor tumorigenic. From this cell line we have selected two sublines, HuMI-T and HuMI-TTul, reflecting diff...

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Autores principales: Yilmaz, A., Gaide, A. C., Sordat, B., Borbenyi, Z., Lahm, H., Imam, A., Schreyer, M., Odartchenko, N.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1993
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968715/
https://www.ncbi.nlm.nih.gov/pubmed/8217602
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author Yilmaz, A.
Gaide, A. C.
Sordat, B.
Borbenyi, Z.
Lahm, H.
Imam, A.
Schreyer, M.
Odartchenko, N.
author_facet Yilmaz, A.
Gaide, A. C.
Sordat, B.
Borbenyi, Z.
Lahm, H.
Imam, A.
Schreyer, M.
Odartchenko, N.
author_sort Yilmaz, A.
collection PubMed
description A human breast epithelial cell line (Hu-MI), established by microinjecting SV40 DNA into human milk epithelial cells, exhibits the phenotype of luminal epithelial cells and is neither clonogenic nor tumorigenic. From this cell line we have selected two sublines, HuMI-T and HuMI-TTul, reflecting different stages of spontaneous transformation. HuMI-T cells grow anchorage-independently, but do not induce tumours in nude mice. HuMI-TTul cells are clonogenic as well as tumorigenic. Cells from both lines exhibit polymorphic structural and numerical chromosome aberrations. Immortalisation of normal luminal epithelial cells from human mammary gland with SV40 DNA alone may thus cause random genetic changes eventually resulting in tumorigenic cell lines. Since Hu-MI, HuMI-T and HuMI-TTul represent some of the consecutive stages taking place during cellular transformation, they are particularly suited as a novel in vitro model system to study progression of human breast cancer. IMAGES:
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spelling pubmed-19687152009-09-10 Malignant progression of SV40-immortalised human milk epithelial cells. Yilmaz, A. Gaide, A. C. Sordat, B. Borbenyi, Z. Lahm, H. Imam, A. Schreyer, M. Odartchenko, N. Br J Cancer Research Article A human breast epithelial cell line (Hu-MI), established by microinjecting SV40 DNA into human milk epithelial cells, exhibits the phenotype of luminal epithelial cells and is neither clonogenic nor tumorigenic. From this cell line we have selected two sublines, HuMI-T and HuMI-TTul, reflecting different stages of spontaneous transformation. HuMI-T cells grow anchorage-independently, but do not induce tumours in nude mice. HuMI-TTul cells are clonogenic as well as tumorigenic. Cells from both lines exhibit polymorphic structural and numerical chromosome aberrations. Immortalisation of normal luminal epithelial cells from human mammary gland with SV40 DNA alone may thus cause random genetic changes eventually resulting in tumorigenic cell lines. Since Hu-MI, HuMI-T and HuMI-TTul represent some of the consecutive stages taking place during cellular transformation, they are particularly suited as a novel in vitro model system to study progression of human breast cancer. IMAGES: Nature Publishing Group 1993-11 /pmc/articles/PMC1968715/ /pubmed/8217602 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Yilmaz, A.
Gaide, A. C.
Sordat, B.
Borbenyi, Z.
Lahm, H.
Imam, A.
Schreyer, M.
Odartchenko, N.
Malignant progression of SV40-immortalised human milk epithelial cells.
title Malignant progression of SV40-immortalised human milk epithelial cells.
title_full Malignant progression of SV40-immortalised human milk epithelial cells.
title_fullStr Malignant progression of SV40-immortalised human milk epithelial cells.
title_full_unstemmed Malignant progression of SV40-immortalised human milk epithelial cells.
title_short Malignant progression of SV40-immortalised human milk epithelial cells.
title_sort malignant progression of sv40-immortalised human milk epithelial cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968715/
https://www.ncbi.nlm.nih.gov/pubmed/8217602
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