Problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation.

The availability of antibodies which recognise p53 protein in paraffin-embedded tissue has created the opportunity to use immunohistochemistry to study the expression of p53 in a wide variety of clinical material. In this paper we have investigated the relationship between the type of fixative and t...

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Autores principales: Fisher, C. J., Gillett, C. E., Vojtĕsek, B., Barnes, D. M., Millis, R. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968757/
https://www.ncbi.nlm.nih.gov/pubmed/7506924
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author Fisher, C. J.
Gillett, C. E.
Vojtĕsek, B.
Barnes, D. M.
Millis, R. R.
author_facet Fisher, C. J.
Gillett, C. E.
Vojtĕsek, B.
Barnes, D. M.
Millis, R. R.
author_sort Fisher, C. J.
collection PubMed
description The availability of antibodies which recognise p53 protein in paraffin-embedded tissue has created the opportunity to use immunohistochemistry to study the expression of p53 in a wide variety of clinical material. In this paper we have investigated the relationship between the type of fixative and the pattern of p53 staining in mammary carcinoma. Optimal results were obtained from breast tissue fixed in phenol formol saline, methacarn or cold formol saline with positive staining for stabilised p53 protein occurring in 69/95 (73%) cases studied. Care must be taken in the interpretation of these results since positive staining for p53 protein is not always indicative of mutation of the p53 gene. Furthermore, a range of staining patterns is seen in mammary carcinomas, making interpretation difficult. Assessment of staining needs to be standardised in order that different studies can be compared. However, in breast carcinoma, p53 immunohistochemistry appears to give information relating to tumour grade and, independently, to prognosis. IMAGES:
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spelling pubmed-19687572009-09-10 Problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation. Fisher, C. J. Gillett, C. E. Vojtĕsek, B. Barnes, D. M. Millis, R. R. Br J Cancer Research Article The availability of antibodies which recognise p53 protein in paraffin-embedded tissue has created the opportunity to use immunohistochemistry to study the expression of p53 in a wide variety of clinical material. In this paper we have investigated the relationship between the type of fixative and the pattern of p53 staining in mammary carcinoma. Optimal results were obtained from breast tissue fixed in phenol formol saline, methacarn or cold formol saline with positive staining for stabilised p53 protein occurring in 69/95 (73%) cases studied. Care must be taken in the interpretation of these results since positive staining for p53 protein is not always indicative of mutation of the p53 gene. Furthermore, a range of staining patterns is seen in mammary carcinomas, making interpretation difficult. Assessment of staining needs to be standardised in order that different studies can be compared. However, in breast carcinoma, p53 immunohistochemistry appears to give information relating to tumour grade and, independently, to prognosis. IMAGES: Nature Publishing Group 1994-01 /pmc/articles/PMC1968757/ /pubmed/7506924 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Fisher, C. J.
Gillett, C. E.
Vojtĕsek, B.
Barnes, D. M.
Millis, R. R.
Problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation.
title Problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation.
title_full Problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation.
title_fullStr Problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation.
title_full_unstemmed Problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation.
title_short Problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation.
title_sort problems with p53 immunohistochemical staining: the effect of fixation and variation in the methods of evaluation.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968757/
https://www.ncbi.nlm.nih.gov/pubmed/7506924
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