An enzyme-linked immunosorbent assay for hypoxia marker binding in tumours.

An enzyme-linked immunosorbent assay (ELISA) has been developed for measuring the in vivo binding of a hexafluorinated 2-nitroimidazole (CCI-103F) in tumour tissue biopsies. The binding of CCI-103F is believed to reflect the presence of hypoxia in tumours. The ELISA provides a sensitive and convenie...

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Autores principales: Raleigh, J. A., La Dine, J. K., Cline, J. M., Thrall, D. E.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968774/
https://www.ncbi.nlm.nih.gov/pubmed/8286212
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author Raleigh, J. A.
La Dine, J. K.
Cline, J. M.
Thrall, D. E.
author_facet Raleigh, J. A.
La Dine, J. K.
Cline, J. M.
Thrall, D. E.
author_sort Raleigh, J. A.
collection PubMed
description An enzyme-linked immunosorbent assay (ELISA) has been developed for measuring the in vivo binding of a hexafluorinated 2-nitroimidazole (CCI-103F) in tumour tissue biopsies. The binding of CCI-103F is believed to reflect the presence of hypoxia in tumours. The ELISA provides a sensitive and convenient method of measuring CCI-103F binding which does not require the injection of radioactive reagents. The ELISA is based on reagents prepared from synthetic antigens formed by the reductive activation and binding of CCI-103F to proteins in novel test tube experiments. Calibration of the ELISA involved comparing the ELISA with the radioactivity contained either in protein-CCI-103F adducts formed in vitro with tritiated CCI-103F or in tissues isolated from a tumour-bearing dog which had been injected with tritium-labelled CCI-103F. The two approaches to calibration are compared. The scope and limitation of the ELISA for measuring the binding of CCI-103F is discussed and an example of the application of the ELISA to measuring changes in tumour hypoxia in canine patients undergoing fractionated radiation therapy is presented. IMAGES:
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spelling pubmed-19687742009-09-10 An enzyme-linked immunosorbent assay for hypoxia marker binding in tumours. Raleigh, J. A. La Dine, J. K. Cline, J. M. Thrall, D. E. Br J Cancer Research Article An enzyme-linked immunosorbent assay (ELISA) has been developed for measuring the in vivo binding of a hexafluorinated 2-nitroimidazole (CCI-103F) in tumour tissue biopsies. The binding of CCI-103F is believed to reflect the presence of hypoxia in tumours. The ELISA provides a sensitive and convenient method of measuring CCI-103F binding which does not require the injection of radioactive reagents. The ELISA is based on reagents prepared from synthetic antigens formed by the reductive activation and binding of CCI-103F to proteins in novel test tube experiments. Calibration of the ELISA involved comparing the ELISA with the radioactivity contained either in protein-CCI-103F adducts formed in vitro with tritiated CCI-103F or in tissues isolated from a tumour-bearing dog which had been injected with tritium-labelled CCI-103F. The two approaches to calibration are compared. The scope and limitation of the ELISA for measuring the binding of CCI-103F is discussed and an example of the application of the ELISA to measuring changes in tumour hypoxia in canine patients undergoing fractionated radiation therapy is presented. IMAGES: Nature Publishing Group 1994-01 /pmc/articles/PMC1968774/ /pubmed/8286212 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Raleigh, J. A.
La Dine, J. K.
Cline, J. M.
Thrall, D. E.
An enzyme-linked immunosorbent assay for hypoxia marker binding in tumours.
title An enzyme-linked immunosorbent assay for hypoxia marker binding in tumours.
title_full An enzyme-linked immunosorbent assay for hypoxia marker binding in tumours.
title_fullStr An enzyme-linked immunosorbent assay for hypoxia marker binding in tumours.
title_full_unstemmed An enzyme-linked immunosorbent assay for hypoxia marker binding in tumours.
title_short An enzyme-linked immunosorbent assay for hypoxia marker binding in tumours.
title_sort enzyme-linked immunosorbent assay for hypoxia marker binding in tumours.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968774/
https://www.ncbi.nlm.nih.gov/pubmed/8286212
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