Expression of transforming growth factor beta mRNA isoforms in human breast cancer.

Using an RNAse protection assay, expression of messenger RNA for isoforms of TGF-beta was determined in a series of breast cancers. Of 50 tumours, 45 (90%) expressed TGF-beta 1 mRNA, 39 (78%) expressed TGF-beta 2, and 47 (94%) expressed TGF-beta 3. Patterns of expression varied between different tum...

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Autores principales: MacCallum, J., Bartlett, J. M., Thompson, A. M., Keen, J. C., Dixon, J. M., Miller, W. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1969445/
https://www.ncbi.nlm.nih.gov/pubmed/8198961
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author MacCallum, J.
Bartlett, J. M.
Thompson, A. M.
Keen, J. C.
Dixon, J. M.
Miller, W. R.
author_facet MacCallum, J.
Bartlett, J. M.
Thompson, A. M.
Keen, J. C.
Dixon, J. M.
Miller, W. R.
author_sort MacCallum, J.
collection PubMed
description Using an RNAse protection assay, expression of messenger RNA for isoforms of TGF-beta was determined in a series of breast cancers. Of 50 tumours, 45 (90%) expressed TGF-beta 1 mRNA, 39 (78%) expressed TGF-beta 2, and 47 (94%) expressed TGF-beta 3. Patterns of expression varied between different tumours: 37 (74%) cancers expressed all three TGF-beta isoforms, ten (20%) expressed only two isoforms and two expressed TGF-beta 1 alone. One sample showed no evidence of TGF-beta mRNA expression. Although most breast cancers expressed mRNA for at least one isoform of TGF-beta, there were differences in patterns of mRNA expression between individual tumours. The relatively small number of tumours examined precluded detailed analysis between expression and other clinical parameters, but a significant association was identified between one aspect of isoform expression and lymph node status, in that the majority of tumours expressing all three isoforms were associated with lymph node involvement, whereas tumours without one or more isoform were usually lymph node negative (P = 0.025 by Fisher's exact test). IMAGES:
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spelling pubmed-19694452009-09-10 Expression of transforming growth factor beta mRNA isoforms in human breast cancer. MacCallum, J. Bartlett, J. M. Thompson, A. M. Keen, J. C. Dixon, J. M. Miller, W. R. Br J Cancer Research Article Using an RNAse protection assay, expression of messenger RNA for isoforms of TGF-beta was determined in a series of breast cancers. Of 50 tumours, 45 (90%) expressed TGF-beta 1 mRNA, 39 (78%) expressed TGF-beta 2, and 47 (94%) expressed TGF-beta 3. Patterns of expression varied between different tumours: 37 (74%) cancers expressed all three TGF-beta isoforms, ten (20%) expressed only two isoforms and two expressed TGF-beta 1 alone. One sample showed no evidence of TGF-beta mRNA expression. Although most breast cancers expressed mRNA for at least one isoform of TGF-beta, there were differences in patterns of mRNA expression between individual tumours. The relatively small number of tumours examined precluded detailed analysis between expression and other clinical parameters, but a significant association was identified between one aspect of isoform expression and lymph node status, in that the majority of tumours expressing all three isoforms were associated with lymph node involvement, whereas tumours without one or more isoform were usually lymph node negative (P = 0.025 by Fisher's exact test). IMAGES: Nature Publishing Group 1994-06 /pmc/articles/PMC1969445/ /pubmed/8198961 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
MacCallum, J.
Bartlett, J. M.
Thompson, A. M.
Keen, J. C.
Dixon, J. M.
Miller, W. R.
Expression of transforming growth factor beta mRNA isoforms in human breast cancer.
title Expression of transforming growth factor beta mRNA isoforms in human breast cancer.
title_full Expression of transforming growth factor beta mRNA isoforms in human breast cancer.
title_fullStr Expression of transforming growth factor beta mRNA isoforms in human breast cancer.
title_full_unstemmed Expression of transforming growth factor beta mRNA isoforms in human breast cancer.
title_short Expression of transforming growth factor beta mRNA isoforms in human breast cancer.
title_sort expression of transforming growth factor beta mrna isoforms in human breast cancer.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1969445/
https://www.ncbi.nlm.nih.gov/pubmed/8198961
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