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The relationship between serum p53 autoantibodies and characteristics of human breast cancer.
Sera from 182 newly diagnosed breast cancer patients were assayed for antibodies to p53 using an enzyme-linked immunosorbent assay (ELISA) method, and antibodies were detected in 48 (26%) compared with 1 out of 76 (1.3%) normal control volunteers (P = 0.0001). In breast cancer patients, autoantibodi...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1994
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1969453/ https://www.ncbi.nlm.nih.gov/pubmed/8198980 |
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author | Mudenda, B. Green, J. A. Green, B. Jenkins, J. R. Robertson, L. Tarunina, M. Leinster, S. J. |
author_facet | Mudenda, B. Green, J. A. Green, B. Jenkins, J. R. Robertson, L. Tarunina, M. Leinster, S. J. |
author_sort | Mudenda, B. |
collection | PubMed |
description | Sera from 182 newly diagnosed breast cancer patients were assayed for antibodies to p53 using an enzyme-linked immunosorbent assay (ELISA) method, and antibodies were detected in 48 (26%) compared with 1 out of 76 (1.3%) normal control volunteers (P = 0.0001). In breast cancer patients, autoantibodies were found in all stages of disease progression: carcinoma in situ, primary invasive breast cancer and in metastatic disease. In the subset of patients in whom sequential sera were assessed over a 6 month period, changes in the p53 antibody titres were observed. The presence of antibodies to p53 correlated positively with high histological grade (P = 0.0012) and a history of second primary cancer (six positive out of eight cases). The incidence of autoantibodies was lower in those patients with a first-degree relative with breast cancer (P = 0.046). Out of 68 patients, there was a significant correlation between positive p53 autoantibody status and the detection of p53 protein in the tissue sections by immunocytochemistry (P = 0.002). In the seronegative patients, positive p53 tumour staining was strongly associated with a family history of breast cancer (P = 0.009). The p53 protein overexpressed in heritable breast cancers may therefore be less immunogenic. The presence of p53 autoantibodies provides important additional information to immunochemistry and may identify patients with aggressive histological types of breast cancer. IMAGES: |
format | Text |
id | pubmed-1969453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1994 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-19694532009-09-10 The relationship between serum p53 autoantibodies and characteristics of human breast cancer. Mudenda, B. Green, J. A. Green, B. Jenkins, J. R. Robertson, L. Tarunina, M. Leinster, S. J. Br J Cancer Research Article Sera from 182 newly diagnosed breast cancer patients were assayed for antibodies to p53 using an enzyme-linked immunosorbent assay (ELISA) method, and antibodies were detected in 48 (26%) compared with 1 out of 76 (1.3%) normal control volunteers (P = 0.0001). In breast cancer patients, autoantibodies were found in all stages of disease progression: carcinoma in situ, primary invasive breast cancer and in metastatic disease. In the subset of patients in whom sequential sera were assessed over a 6 month period, changes in the p53 antibody titres were observed. The presence of antibodies to p53 correlated positively with high histological grade (P = 0.0012) and a history of second primary cancer (six positive out of eight cases). The incidence of autoantibodies was lower in those patients with a first-degree relative with breast cancer (P = 0.046). Out of 68 patients, there was a significant correlation between positive p53 autoantibody status and the detection of p53 protein in the tissue sections by immunocytochemistry (P = 0.002). In the seronegative patients, positive p53 tumour staining was strongly associated with a family history of breast cancer (P = 0.009). The p53 protein overexpressed in heritable breast cancers may therefore be less immunogenic. The presence of p53 autoantibodies provides important additional information to immunochemistry and may identify patients with aggressive histological types of breast cancer. IMAGES: Nature Publishing Group 1994-06 /pmc/articles/PMC1969453/ /pubmed/8198980 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Mudenda, B. Green, J. A. Green, B. Jenkins, J. R. Robertson, L. Tarunina, M. Leinster, S. J. The relationship between serum p53 autoantibodies and characteristics of human breast cancer. |
title | The relationship between serum p53 autoantibodies and characteristics of human breast cancer. |
title_full | The relationship between serum p53 autoantibodies and characteristics of human breast cancer. |
title_fullStr | The relationship between serum p53 autoantibodies and characteristics of human breast cancer. |
title_full_unstemmed | The relationship between serum p53 autoantibodies and characteristics of human breast cancer. |
title_short | The relationship between serum p53 autoantibodies and characteristics of human breast cancer. |
title_sort | relationship between serum p53 autoantibodies and characteristics of human breast cancer. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1969453/ https://www.ncbi.nlm.nih.gov/pubmed/8198980 |
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