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Low incidence of ras oncogene activation in human squamous cell carcinomas.
Activation of the ras gene family by point mutation at codons 12, 13 and 61 has been demonstrated in up to 20% of unselected series of human tumours. The present study was carried out to assess the incidence of ras activation in 37 squamous cell carcinomas of the head and neck, seven squamous cell c...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1990
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971288/ https://www.ncbi.nlm.nih.gov/pubmed/2183872 |
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author | Rumsby, G. Carter, R. L. Gusterson, B. A. |
author_facet | Rumsby, G. Carter, R. L. Gusterson, B. A. |
author_sort | Rumsby, G. |
collection | PubMed |
description | Activation of the ras gene family by point mutation at codons 12, 13 and 61 has been demonstrated in up to 20% of unselected series of human tumours. The present study was carried out to assess the incidence of ras activation in 37 squamous cell carcinomas of the head and neck, seven squamous cell carcinomas of the skin and eight squamous carcinoma cell lines. Oligonucleotide probes and the polymerase chain reaction were used on DNA extracted from achival paraffin embedded material. Mutations in codon 12 of the Harvey ras gene was found in a carcinoma of the larynx and a carcinoma of the lip, both of which had received prior irradiation. A cell line (LICR-LON-HN8) established from the same laryngeal cancer showed the same mutation. This study indicates that there is a low incidence of ras mutation in human squamous cell carcinomas and that activation of this family of genes is probably not a common factor in the development of this group of tumours. IMAGES: |
format | Text |
id | pubmed-1971288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1990 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-19712882009-09-10 Low incidence of ras oncogene activation in human squamous cell carcinomas. Rumsby, G. Carter, R. L. Gusterson, B. A. Br J Cancer Research Article Activation of the ras gene family by point mutation at codons 12, 13 and 61 has been demonstrated in up to 20% of unselected series of human tumours. The present study was carried out to assess the incidence of ras activation in 37 squamous cell carcinomas of the head and neck, seven squamous cell carcinomas of the skin and eight squamous carcinoma cell lines. Oligonucleotide probes and the polymerase chain reaction were used on DNA extracted from achival paraffin embedded material. Mutations in codon 12 of the Harvey ras gene was found in a carcinoma of the larynx and a carcinoma of the lip, both of which had received prior irradiation. A cell line (LICR-LON-HN8) established from the same laryngeal cancer showed the same mutation. This study indicates that there is a low incidence of ras mutation in human squamous cell carcinomas and that activation of this family of genes is probably not a common factor in the development of this group of tumours. IMAGES: Nature Publishing Group 1990-03 /pmc/articles/PMC1971288/ /pubmed/2183872 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Rumsby, G. Carter, R. L. Gusterson, B. A. Low incidence of ras oncogene activation in human squamous cell carcinomas. |
title | Low incidence of ras oncogene activation in human squamous cell carcinomas. |
title_full | Low incidence of ras oncogene activation in human squamous cell carcinomas. |
title_fullStr | Low incidence of ras oncogene activation in human squamous cell carcinomas. |
title_full_unstemmed | Low incidence of ras oncogene activation in human squamous cell carcinomas. |
title_short | Low incidence of ras oncogene activation in human squamous cell carcinomas. |
title_sort | low incidence of ras oncogene activation in human squamous cell carcinomas. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971288/ https://www.ncbi.nlm.nih.gov/pubmed/2183872 |
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