Detection of the growth fraction in colorectal tumours by a monoclonal antibody against DNA polymerase alpha.

The cell kinetics of 54 colorectal tumours were examined by immunohistochemical methods, using the monoclonal antibody DNA polymerase alpha which reacts with an antigen found only in proliferating cells. The rate of DNA polymerase alpha positive cells in colorectal cancer was 44.8%, a figure that wa...

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Autores principales: Yamaguchi, A., Takegawa, S., Ishida, T., Nishimura, G., Kato, M., Kanno, M., Kosaka, T., Yonemura, Y., Miyazaki, I.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1990
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971304/
https://www.ncbi.nlm.nih.gov/pubmed/2328204
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author Yamaguchi, A.
Takegawa, S.
Ishida, T.
Nishimura, G.
Kato, M.
Kanno, M.
Kosaka, T.
Yonemura, Y.
Miyazaki, I.
author_facet Yamaguchi, A.
Takegawa, S.
Ishida, T.
Nishimura, G.
Kato, M.
Kanno, M.
Kosaka, T.
Yonemura, Y.
Miyazaki, I.
author_sort Yamaguchi, A.
collection PubMed
description The cell kinetics of 54 colorectal tumours were examined by immunohistochemical methods, using the monoclonal antibody DNA polymerase alpha which reacts with an antigen found only in proliferating cells. The rate of DNA polymerase alpha positive cells in colorectal cancer was 44.8%, a figure that was significantly higher than the 21.9% found in colorectal adenomas. The rate of DNA polymerase alpha positive cells tended to rise as the degree of differentiation decreased according to the standard histological grading criteria for colorectal cancer. Positive cells were detected in much greater numbers in tumours with liver metastasis (55.4%) than in those without metastasis (41.7%). The rate of DNA polymerase alpha positive cells for aneuploid lesions was higher than that for lesions with a diploid pattern. The determination of growth fractions with a monoclonal antibody (DNA polymerase alpha) may be a biological marker of great prognostic significance. IMAGES:
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spelling pubmed-19713042009-09-10 Detection of the growth fraction in colorectal tumours by a monoclonal antibody against DNA polymerase alpha. Yamaguchi, A. Takegawa, S. Ishida, T. Nishimura, G. Kato, M. Kanno, M. Kosaka, T. Yonemura, Y. Miyazaki, I. Br J Cancer Research Article The cell kinetics of 54 colorectal tumours were examined by immunohistochemical methods, using the monoclonal antibody DNA polymerase alpha which reacts with an antigen found only in proliferating cells. The rate of DNA polymerase alpha positive cells in colorectal cancer was 44.8%, a figure that was significantly higher than the 21.9% found in colorectal adenomas. The rate of DNA polymerase alpha positive cells tended to rise as the degree of differentiation decreased according to the standard histological grading criteria for colorectal cancer. Positive cells were detected in much greater numbers in tumours with liver metastasis (55.4%) than in those without metastasis (41.7%). The rate of DNA polymerase alpha positive cells for aneuploid lesions was higher than that for lesions with a diploid pattern. The determination of growth fractions with a monoclonal antibody (DNA polymerase alpha) may be a biological marker of great prognostic significance. IMAGES: Nature Publishing Group 1990-03 /pmc/articles/PMC1971304/ /pubmed/2328204 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Yamaguchi, A.
Takegawa, S.
Ishida, T.
Nishimura, G.
Kato, M.
Kanno, M.
Kosaka, T.
Yonemura, Y.
Miyazaki, I.
Detection of the growth fraction in colorectal tumours by a monoclonal antibody against DNA polymerase alpha.
title Detection of the growth fraction in colorectal tumours by a monoclonal antibody against DNA polymerase alpha.
title_full Detection of the growth fraction in colorectal tumours by a monoclonal antibody against DNA polymerase alpha.
title_fullStr Detection of the growth fraction in colorectal tumours by a monoclonal antibody against DNA polymerase alpha.
title_full_unstemmed Detection of the growth fraction in colorectal tumours by a monoclonal antibody against DNA polymerase alpha.
title_short Detection of the growth fraction in colorectal tumours by a monoclonal antibody against DNA polymerase alpha.
title_sort detection of the growth fraction in colorectal tumours by a monoclonal antibody against dna polymerase alpha.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971304/
https://www.ncbi.nlm.nih.gov/pubmed/2328204
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