P-glycoprotein expression in primary breast cancer detected by immunocytochemistry with two monoclonal antibodies.

We have investigated P-glycoprotein (P-gp) expression in samples of primary breast cancer from 29 patients before therapy. We employed immunohistochemical techniques using two monoclonal antibodies (C219 and MRK16) and an indirect alkaline phosphatase method. Heterogeneous expression in epithelial c...

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Detalles Bibliográficos
Autores principales: Wishart, G. C., Plumb, J. A., Going, J. J., McNicol, A. M., McArdle, C. S., Tsuruo, T., Kaye, S. B.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1990
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971543/
https://www.ncbi.nlm.nih.gov/pubmed/1978783
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author Wishart, G. C.
Plumb, J. A.
Going, J. J.
McNicol, A. M.
McArdle, C. S.
Tsuruo, T.
Kaye, S. B.
author_facet Wishart, G. C.
Plumb, J. A.
Going, J. J.
McNicol, A. M.
McArdle, C. S.
Tsuruo, T.
Kaye, S. B.
author_sort Wishart, G. C.
collection PubMed
description We have investigated P-glycoprotein (P-gp) expression in samples of primary breast cancer from 29 patients before therapy. We employed immunohistochemical techniques using two monoclonal antibodies (C219 and MRK16) and an indirect alkaline phosphatase method. Heterogeneous expression in epithelial cells was detected with both C219 (21 of 29) and MRK16 (16 of 29). A surprising finding was P-glycoprotein expression in stromal cells with both C219 (26 of 29) and MRK16 (12 of 29). Our results suggest that significant levels of P-glycoprotein expression may be present in breast cancer before exposure to drugs associated with multidrug resistance. IMAGES:
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spelling pubmed-19715432009-09-10 P-glycoprotein expression in primary breast cancer detected by immunocytochemistry with two monoclonal antibodies. Wishart, G. C. Plumb, J. A. Going, J. J. McNicol, A. M. McArdle, C. S. Tsuruo, T. Kaye, S. B. Br J Cancer Research Article We have investigated P-glycoprotein (P-gp) expression in samples of primary breast cancer from 29 patients before therapy. We employed immunohistochemical techniques using two monoclonal antibodies (C219 and MRK16) and an indirect alkaline phosphatase method. Heterogeneous expression in epithelial cells was detected with both C219 (21 of 29) and MRK16 (16 of 29). A surprising finding was P-glycoprotein expression in stromal cells with both C219 (26 of 29) and MRK16 (12 of 29). Our results suggest that significant levels of P-glycoprotein expression may be present in breast cancer before exposure to drugs associated with multidrug resistance. IMAGES: Nature Publishing Group 1990-11 /pmc/articles/PMC1971543/ /pubmed/1978783 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Wishart, G. C.
Plumb, J. A.
Going, J. J.
McNicol, A. M.
McArdle, C. S.
Tsuruo, T.
Kaye, S. B.
P-glycoprotein expression in primary breast cancer detected by immunocytochemistry with two monoclonal antibodies.
title P-glycoprotein expression in primary breast cancer detected by immunocytochemistry with two monoclonal antibodies.
title_full P-glycoprotein expression in primary breast cancer detected by immunocytochemistry with two monoclonal antibodies.
title_fullStr P-glycoprotein expression in primary breast cancer detected by immunocytochemistry with two monoclonal antibodies.
title_full_unstemmed P-glycoprotein expression in primary breast cancer detected by immunocytochemistry with two monoclonal antibodies.
title_short P-glycoprotein expression in primary breast cancer detected by immunocytochemistry with two monoclonal antibodies.
title_sort p-glycoprotein expression in primary breast cancer detected by immunocytochemistry with two monoclonal antibodies.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971543/
https://www.ncbi.nlm.nih.gov/pubmed/1978783
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