Cargando…
Characterisation of adriamycin- and amsacrine-resistant human leukaemic T cell lines.
Cell lines resistant to adriamycin and amsacrine were derived from cloned sublines of the human T cell line Jurkat. Most of the lines resemble atypical MDR cells (Danks et al., 1987; Beck et al., 1987). Thus, resistant Jurkat sublines were cross resistant to several topoisomerase II inhibiting drugs...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1991
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971666/ https://www.ncbi.nlm.nih.gov/pubmed/1989661 |
_version_ | 1782134959572516864 |
---|---|
author | Snow, K. Judd, W. |
author_facet | Snow, K. Judd, W. |
author_sort | Snow, K. |
collection | PubMed |
description | Cell lines resistant to adriamycin and amsacrine were derived from cloned sublines of the human T cell line Jurkat. Most of the lines resemble atypical MDR cells (Danks et al., 1987; Beck et al., 1987). Thus, resistant Jurkat sublines were cross resistant to several topoisomerase II inhibiting drugs but had low or no resistance to other classes of drugs, resistance was not reversed by verapamil, Pgp was not overexpressed, and drug accumulation was unaltered in resistant compared to parental (control) sublines. Other findings were that anthracycline metabolism differed between resistant and parental sublines, and that resistant sublines displayed altered expression of small polypeptides (less than 20K MW) and an 85K MW protein. Drug resistant cells showed resistance to the production of drug induced cytogenetic aberrations, DNA breaks, and protein-DNA complexes. Resistance was not mediated by altered binding of drugs to DNA or by increased repair of DNA damage. Indirect evidence suggests that the resistant cells had an altered drug-DNA-topoisomerase II association. The study highlights the complex relationships between DNA breaks, cytogenetic aberrations, protein-DNA complexes and drug cytotoxicity, and shows that the relationships differ for adriamycin and amsacrine, suggesting some differences in the modes of action and/or resistance for the drugs and cell lines. IMAGES: |
format | Text |
id | pubmed-1971666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1991 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-19716662009-09-10 Characterisation of adriamycin- and amsacrine-resistant human leukaemic T cell lines. Snow, K. Judd, W. Br J Cancer Research Article Cell lines resistant to adriamycin and amsacrine were derived from cloned sublines of the human T cell line Jurkat. Most of the lines resemble atypical MDR cells (Danks et al., 1987; Beck et al., 1987). Thus, resistant Jurkat sublines were cross resistant to several topoisomerase II inhibiting drugs but had low or no resistance to other classes of drugs, resistance was not reversed by verapamil, Pgp was not overexpressed, and drug accumulation was unaltered in resistant compared to parental (control) sublines. Other findings were that anthracycline metabolism differed between resistant and parental sublines, and that resistant sublines displayed altered expression of small polypeptides (less than 20K MW) and an 85K MW protein. Drug resistant cells showed resistance to the production of drug induced cytogenetic aberrations, DNA breaks, and protein-DNA complexes. Resistance was not mediated by altered binding of drugs to DNA or by increased repair of DNA damage. Indirect evidence suggests that the resistant cells had an altered drug-DNA-topoisomerase II association. The study highlights the complex relationships between DNA breaks, cytogenetic aberrations, protein-DNA complexes and drug cytotoxicity, and shows that the relationships differ for adriamycin and amsacrine, suggesting some differences in the modes of action and/or resistance for the drugs and cell lines. IMAGES: Nature Publishing Group 1991-01 /pmc/articles/PMC1971666/ /pubmed/1989661 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Snow, K. Judd, W. Characterisation of adriamycin- and amsacrine-resistant human leukaemic T cell lines. |
title | Characterisation of adriamycin- and amsacrine-resistant human leukaemic T cell lines. |
title_full | Characterisation of adriamycin- and amsacrine-resistant human leukaemic T cell lines. |
title_fullStr | Characterisation of adriamycin- and amsacrine-resistant human leukaemic T cell lines. |
title_full_unstemmed | Characterisation of adriamycin- and amsacrine-resistant human leukaemic T cell lines. |
title_short | Characterisation of adriamycin- and amsacrine-resistant human leukaemic T cell lines. |
title_sort | characterisation of adriamycin- and amsacrine-resistant human leukaemic t cell lines. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971666/ https://www.ncbi.nlm.nih.gov/pubmed/1989661 |
work_keys_str_mv | AT snowk characterisationofadriamycinandamsacrineresistanthumanleukaemictcelllines AT juddw characterisationofadriamycinandamsacrineresistanthumanleukaemictcelllines |