Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma.

A new cell line (CAL51) was isolated from a malignant pleural effusion of a woman with metastatic breast cancer. These cells grow in continuous culture and exhibit the morphological, ultrastructural and immunohistochemical features of epithelial cells of mammary origin. They are tumorigenic in nude...

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Autores principales: Gioanni, J., Le François, D., Zanghellini, E., Mazeau, C., Ettore, F., Lambert, J. C., Schneider, M., Dutrillaux, B.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1990
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971752/
https://www.ncbi.nlm.nih.gov/pubmed/2390488
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author Gioanni, J.
Le François, D.
Zanghellini, E.
Mazeau, C.
Ettore, F.
Lambert, J. C.
Schneider, M.
Dutrillaux, B.
author_facet Gioanni, J.
Le François, D.
Zanghellini, E.
Mazeau, C.
Ettore, F.
Lambert, J. C.
Schneider, M.
Dutrillaux, B.
author_sort Gioanni, J.
collection PubMed
description A new cell line (CAL51) was isolated from a malignant pleural effusion of a woman with metastatic breast cancer. These cells grow in continuous culture and exhibit the morphological, ultrastructural and immunohistochemical features of epithelial cells of mammary origin. They are tumorigenic in nude mice and clone in soft agar. Oestrogen receptors are not detected. CAL51 consists of a homogeneous population of cells with normal chromosomes even after the use of high resolution banding. Cytogenetic analysis of the cells from the tumour induced by CAL51 in the nude mouse confirmed the normality and the stability of the karyotype. All breast cancer cell lines established to date present abnormal karyotypes; CAL51 cell line may be more informative than cell lines with aberrant karyotypes for investigating essential genetic differences between normal and malignant mammary gland cells. IMAGES:
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spelling pubmed-19717522009-09-10 Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma. Gioanni, J. Le François, D. Zanghellini, E. Mazeau, C. Ettore, F. Lambert, J. C. Schneider, M. Dutrillaux, B. Br J Cancer Research Article A new cell line (CAL51) was isolated from a malignant pleural effusion of a woman with metastatic breast cancer. These cells grow in continuous culture and exhibit the morphological, ultrastructural and immunohistochemical features of epithelial cells of mammary origin. They are tumorigenic in nude mice and clone in soft agar. Oestrogen receptors are not detected. CAL51 consists of a homogeneous population of cells with normal chromosomes even after the use of high resolution banding. Cytogenetic analysis of the cells from the tumour induced by CAL51 in the nude mouse confirmed the normality and the stability of the karyotype. All breast cancer cell lines established to date present abnormal karyotypes; CAL51 cell line may be more informative than cell lines with aberrant karyotypes for investigating essential genetic differences between normal and malignant mammary gland cells. IMAGES: Nature Publishing Group 1990-07 /pmc/articles/PMC1971752/ /pubmed/2390488 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Gioanni, J.
Le François, D.
Zanghellini, E.
Mazeau, C.
Ettore, F.
Lambert, J. C.
Schneider, M.
Dutrillaux, B.
Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma.
title Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma.
title_full Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma.
title_fullStr Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma.
title_full_unstemmed Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma.
title_short Establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma.
title_sort establishment and characterisation of a new tumorigenic cell line with a normal karyotype derived from a human breast adenocarcinoma.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971752/
https://www.ncbi.nlm.nih.gov/pubmed/2390488
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