Intermittent blood flow in the KHT sarcoma--flow cytometry studies using Hoechst 33342.

The administration of the fluorescent DNA stain, Hoechst 33342, to mice bearing the KHT sarcoma, combined with flow cytometry, can be used to select cells according to their proximity to functional vasculature. Different protocols of administration of Hoechst 33342 were used in order to differentiat...

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Autores principales: Minchinton, A. I., Durand, R. E., Chaplin, D. J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1990
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971840/
https://www.ncbi.nlm.nih.gov/pubmed/2386734
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author Minchinton, A. I.
Durand, R. E.
Chaplin, D. J.
author_facet Minchinton, A. I.
Durand, R. E.
Chaplin, D. J.
author_sort Minchinton, A. I.
collection PubMed
description The administration of the fluorescent DNA stain, Hoechst 33342, to mice bearing the KHT sarcoma, combined with flow cytometry, can be used to select cells according to their proximity to functional vasculature. Different protocols of administration of Hoechst 33342 were used in order to differentiate between the presence of temporary and chronically hypoxic cells. The results show a large difference in radiosensitivity between cells close to, and distant from, functional vasculature. However, this pattern of radiosensitivity is observed only when the staining period with Hoechst 33342 is short and coincides with the period of irradiation. When the radiation treatment is temporally divorced from the staining period then the radiosensitivity and staining intensity are not related. This result can be interpreted as indicating that hypoxic cells exist within this tumour as a result of fluctuations in tumour blood flow.
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spelling pubmed-19718402009-09-10 Intermittent blood flow in the KHT sarcoma--flow cytometry studies using Hoechst 33342. Minchinton, A. I. Durand, R. E. Chaplin, D. J. Br J Cancer Research Article The administration of the fluorescent DNA stain, Hoechst 33342, to mice bearing the KHT sarcoma, combined with flow cytometry, can be used to select cells according to their proximity to functional vasculature. Different protocols of administration of Hoechst 33342 were used in order to differentiate between the presence of temporary and chronically hypoxic cells. The results show a large difference in radiosensitivity between cells close to, and distant from, functional vasculature. However, this pattern of radiosensitivity is observed only when the staining period with Hoechst 33342 is short and coincides with the period of irradiation. When the radiation treatment is temporally divorced from the staining period then the radiosensitivity and staining intensity are not related. This result can be interpreted as indicating that hypoxic cells exist within this tumour as a result of fluctuations in tumour blood flow. Nature Publishing Group 1990-08 /pmc/articles/PMC1971840/ /pubmed/2386734 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Minchinton, A. I.
Durand, R. E.
Chaplin, D. J.
Intermittent blood flow in the KHT sarcoma--flow cytometry studies using Hoechst 33342.
title Intermittent blood flow in the KHT sarcoma--flow cytometry studies using Hoechst 33342.
title_full Intermittent blood flow in the KHT sarcoma--flow cytometry studies using Hoechst 33342.
title_fullStr Intermittent blood flow in the KHT sarcoma--flow cytometry studies using Hoechst 33342.
title_full_unstemmed Intermittent blood flow in the KHT sarcoma--flow cytometry studies using Hoechst 33342.
title_short Intermittent blood flow in the KHT sarcoma--flow cytometry studies using Hoechst 33342.
title_sort intermittent blood flow in the kht sarcoma--flow cytometry studies using hoechst 33342.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1971840/
https://www.ncbi.nlm.nih.gov/pubmed/2386734
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