A comparison of in vivo and in vitro 31P NMR spectra from human breast tumours: variations in phospholipid metabolism.

An in vivo 31P NMR spectrum was obtained from each of four human breast tumours. The phosphomonoester and phosphodiester region of each spectrum consisted of a broad peak. Chemical extracts from samples of each of the tumours obtained at resection were examined on a high field strength NMR system. T...

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Autores principales: Smith, T. A., Glaholm, J., Leach, M. O., Machin, L., Collins, D. J., Payne, G. S., McCready, V. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1991
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1972336/
https://www.ncbi.nlm.nih.gov/pubmed/2021535
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author Smith, T. A.
Glaholm, J.
Leach, M. O.
Machin, L.
Collins, D. J.
Payne, G. S.
McCready, V. R.
author_facet Smith, T. A.
Glaholm, J.
Leach, M. O.
Machin, L.
Collins, D. J.
Payne, G. S.
McCready, V. R.
author_sort Smith, T. A.
collection PubMed
description An in vivo 31P NMR spectrum was obtained from each of four human breast tumours. The phosphomonoester and phosphodiester region of each spectrum consisted of a broad peak. Chemical extracts from samples of each of the tumours obtained at resection were examined on a high field strength NMR system. The phosphomonoester region in the spectrum from each extract resolved into three peaks consisting of phosphocholine, phosphoethanolamine and a nucleoside monophosphate. The phosphodiester region resolved into two components, glycerophosphorylcholine and glycerophosphorylethanolamine. Comparing the in vivo and in vitro data from each tumour showed that the contribution of phosphodiester was much lower in the in vitro spectra. We believe this to be a consequence of phospholipid, which would not appear in the aqueous extract, contributing to the phosphodiester peak in vivo.
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spelling pubmed-19723362009-09-10 A comparison of in vivo and in vitro 31P NMR spectra from human breast tumours: variations in phospholipid metabolism. Smith, T. A. Glaholm, J. Leach, M. O. Machin, L. Collins, D. J. Payne, G. S. McCready, V. R. Br J Cancer Research Article An in vivo 31P NMR spectrum was obtained from each of four human breast tumours. The phosphomonoester and phosphodiester region of each spectrum consisted of a broad peak. Chemical extracts from samples of each of the tumours obtained at resection were examined on a high field strength NMR system. The phosphomonoester region in the spectrum from each extract resolved into three peaks consisting of phosphocholine, phosphoethanolamine and a nucleoside monophosphate. The phosphodiester region resolved into two components, glycerophosphorylcholine and glycerophosphorylethanolamine. Comparing the in vivo and in vitro data from each tumour showed that the contribution of phosphodiester was much lower in the in vitro spectra. We believe this to be a consequence of phospholipid, which would not appear in the aqueous extract, contributing to the phosphodiester peak in vivo. Nature Publishing Group 1991-04 /pmc/articles/PMC1972336/ /pubmed/2021535 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Smith, T. A.
Glaholm, J.
Leach, M. O.
Machin, L.
Collins, D. J.
Payne, G. S.
McCready, V. R.
A comparison of in vivo and in vitro 31P NMR spectra from human breast tumours: variations in phospholipid metabolism.
title A comparison of in vivo and in vitro 31P NMR spectra from human breast tumours: variations in phospholipid metabolism.
title_full A comparison of in vivo and in vitro 31P NMR spectra from human breast tumours: variations in phospholipid metabolism.
title_fullStr A comparison of in vivo and in vitro 31P NMR spectra from human breast tumours: variations in phospholipid metabolism.
title_full_unstemmed A comparison of in vivo and in vitro 31P NMR spectra from human breast tumours: variations in phospholipid metabolism.
title_short A comparison of in vivo and in vitro 31P NMR spectra from human breast tumours: variations in phospholipid metabolism.
title_sort comparison of in vivo and in vitro 31p nmr spectra from human breast tumours: variations in phospholipid metabolism.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1972336/
https://www.ncbi.nlm.nih.gov/pubmed/2021535
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