Microencapsulation of human cells: its effects on growth of normal and tumour cells in vitro.

The growth kinetics of established human colorectal tumour cell lines (HT29, HT115 and COLO 320DM) and human diploid fibroblasts (Flow 2002) were studied in conventional culture and in microcapsules formed from alginate-poly(L-lysine)-alginate membranes. The tumour lines grew rapidly in microcapsule...

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Detalles Bibliográficos
Autores principales: Shimi, S. M., Hopwood, D., Newman, E. L., Cuschieri, A.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1991
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1972378/
https://www.ncbi.nlm.nih.gov/pubmed/2039691
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author Shimi, S. M.
Hopwood, D.
Newman, E. L.
Cuschieri, A.
author_facet Shimi, S. M.
Hopwood, D.
Newman, E. L.
Cuschieri, A.
author_sort Shimi, S. M.
collection PubMed
description The growth kinetics of established human colorectal tumour cell lines (HT29, HT115 and COLO 320DM) and human diploid fibroblasts (Flow 2002) were studied in conventional culture and in microcapsules formed from alginate-poly(L-lysine)-alginate membranes. The tumour lines grew rapidly in microcapsules but, in the case of the substrate-adherent lines HT29 and HT115, only after a prolonged lag phase. This phase was reduced by serial passage in microcapsules. The anchorage-independent line COLO 320DM showed no lengthening in lag phase. Microencapsulated fibroblasts underwent negligible growth but remained viable. Some evidence for functional differentiation (microvilli, cell-cell junctions) of the tumour line HT115 within the microcapsules was observed. We conclude that the use of microcapsules provides an alternative system with some advantages for the study of human cancer and its metastases in vitro. IMAGES:
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spelling pubmed-19723782009-09-10 Microencapsulation of human cells: its effects on growth of normal and tumour cells in vitro. Shimi, S. M. Hopwood, D. Newman, E. L. Cuschieri, A. Br J Cancer Research Article The growth kinetics of established human colorectal tumour cell lines (HT29, HT115 and COLO 320DM) and human diploid fibroblasts (Flow 2002) were studied in conventional culture and in microcapsules formed from alginate-poly(L-lysine)-alginate membranes. The tumour lines grew rapidly in microcapsules but, in the case of the substrate-adherent lines HT29 and HT115, only after a prolonged lag phase. This phase was reduced by serial passage in microcapsules. The anchorage-independent line COLO 320DM showed no lengthening in lag phase. Microencapsulated fibroblasts underwent negligible growth but remained viable. Some evidence for functional differentiation (microvilli, cell-cell junctions) of the tumour line HT115 within the microcapsules was observed. We conclude that the use of microcapsules provides an alternative system with some advantages for the study of human cancer and its metastases in vitro. IMAGES: Nature Publishing Group 1991-05 /pmc/articles/PMC1972378/ /pubmed/2039691 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Shimi, S. M.
Hopwood, D.
Newman, E. L.
Cuschieri, A.
Microencapsulation of human cells: its effects on growth of normal and tumour cells in vitro.
title Microencapsulation of human cells: its effects on growth of normal and tumour cells in vitro.
title_full Microencapsulation of human cells: its effects on growth of normal and tumour cells in vitro.
title_fullStr Microencapsulation of human cells: its effects on growth of normal and tumour cells in vitro.
title_full_unstemmed Microencapsulation of human cells: its effects on growth of normal and tumour cells in vitro.
title_short Microencapsulation of human cells: its effects on growth of normal and tumour cells in vitro.
title_sort microencapsulation of human cells: its effects on growth of normal and tumour cells in vitro.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1972378/
https://www.ncbi.nlm.nih.gov/pubmed/2039691
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AT newmanel microencapsulationofhumancellsitseffectsongrowthofnormalandtumourcellsinvitro
AT cuschieria microencapsulationofhumancellsitseffectsongrowthofnormalandtumourcellsinvitro

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