Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification.

Two human cell lines (UACC-812 and 893), both containing significant amplification of the HER-2/neu gene, were established from biopsy specimens of breast carcinomas. One patient had Stage II breast carcinoma; the other had metastatic disease. Characterisation of these lines has revealed that both a...

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Autores principales: Meltzer, P., Leibovitz, A., Dalton, W., Villar, H., Kute, T., Davis, J., Nagle, R., Trent, J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1991
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1972383/
https://www.ncbi.nlm.nih.gov/pubmed/1674877
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author Meltzer, P.
Leibovitz, A.
Dalton, W.
Villar, H.
Kute, T.
Davis, J.
Nagle, R.
Trent, J.
author_facet Meltzer, P.
Leibovitz, A.
Dalton, W.
Villar, H.
Kute, T.
Davis, J.
Nagle, R.
Trent, J.
author_sort Meltzer, P.
collection PubMed
description Two human cell lines (UACC-812 and 893), both containing significant amplification of the HER-2/neu gene, were established from biopsy specimens of breast carcinomas. One patient had Stage II breast carcinoma; the other had metastatic disease. Characterisation of these lines has revealed that both are highly aneuploid containing multiple clonal chromosome alterations, have doubling times near 100 h, and are oestrogen and progesterone receptor negative. Electron microscopy demonstrates that both lines contain numerous microvilli, cytoplasmic filaments, multivesicular bodies, and desmosomes. Immunoblot analysis for P-glycoprotein using the monoclonal antibody C219 was negative for both patient cell lines. These relatively rare cell lines may represent a useful model to investigate human breast carcinomas. IMAGES:
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spelling pubmed-19723832009-09-10 Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification. Meltzer, P. Leibovitz, A. Dalton, W. Villar, H. Kute, T. Davis, J. Nagle, R. Trent, J. Br J Cancer Research Article Two human cell lines (UACC-812 and 893), both containing significant amplification of the HER-2/neu gene, were established from biopsy specimens of breast carcinomas. One patient had Stage II breast carcinoma; the other had metastatic disease. Characterisation of these lines has revealed that both are highly aneuploid containing multiple clonal chromosome alterations, have doubling times near 100 h, and are oestrogen and progesterone receptor negative. Electron microscopy demonstrates that both lines contain numerous microvilli, cytoplasmic filaments, multivesicular bodies, and desmosomes. Immunoblot analysis for P-glycoprotein using the monoclonal antibody C219 was negative for both patient cell lines. These relatively rare cell lines may represent a useful model to investigate human breast carcinomas. IMAGES: Nature Publishing Group 1991-05 /pmc/articles/PMC1972383/ /pubmed/1674877 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Meltzer, P.
Leibovitz, A.
Dalton, W.
Villar, H.
Kute, T.
Davis, J.
Nagle, R.
Trent, J.
Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification.
title Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification.
title_full Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification.
title_fullStr Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification.
title_full_unstemmed Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification.
title_short Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification.
title_sort establishment of two new cell lines derived from human breast carcinomas with her-2/neu amplification.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1972383/
https://www.ncbi.nlm.nih.gov/pubmed/1674877
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