Impact of polysialylated CD56 on natural killer cell cytotoxicity

BACKGROUND: Siglec-7, a sialic acid binding inhibitory receptor expressed by NK cells is masked in vivo by a so far unknown ligand. It shows a strong binding prevalence for α-2,8-linked disialic acids in vitro. RESULTS: Here we describe the expression of PSA-NCAM (α-2,8-linked polysialic acid modifi...

Descripción completa

Detalles Bibliográficos
Autores principales: Moebius, Jeannette M, Widera, Darius, Schmitz, Juergen, Kaltschmidt, Christian, Piechaczek, Christoph
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976417/
https://www.ncbi.nlm.nih.gov/pubmed/17683591
http://dx.doi.org/10.1186/1471-2172-8-13
Descripción
Sumario:BACKGROUND: Siglec-7, a sialic acid binding inhibitory receptor expressed by NK cells is masked in vivo by a so far unknown ligand. It shows a strong binding prevalence for α-2,8-linked disialic acids in vitro. RESULTS: Here we describe the expression of PSA-NCAM (α-2,8-linked polysialic acid modified NCAM) on functional adult peripheral blood natural killer cells and examine its possible role in masking Siglec-7. Unmasking of Siglec-7 using Clostridium perfringens neuraminidase massively reduces NK cell cytotoxicity. By contrast a specific removal of PSA using Endo-NF does not lead to a reduction of NK cell cytotoxicity. CONCLUSION: The results presented here therefore indicate that PSA-NCAM is not involved in masking Siglec-7.

Ejemplares similares