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Improved identification of enriched peptide–RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry

Direct UV cross-linking combined with mass spectrometry (MS) is a powerful tool to identify hitherto non-characterized protein–RNA contact sites in native ribonucleoprotein particles (RNPs) such as the spliceosome. Identification of contact sites after cross-linking is restricted by: (i) the relativ...

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Detalles Bibliográficos
Autores principales:	Kühn-Hölsken, Eva, Dybkov, Olexandr, Sander, Björn, Lührmann, Reinhard, Urlaub, Henning
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976460/ https://www.ncbi.nlm.nih.gov/pubmed/17652325 http://dx.doi.org/10.1093/nar/gkm540

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