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Can eukaryotic cells monitor the presence of unreplicated DNA?
Completion of DNA replication before mitosis is essential for genome stability and cell viability. Cellular controls called checkpoints act as surveillance mechanisms capable of detecting errors and blocking cell cycle progression to allow time for those errors to be corrected. An important question...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976610/ https://www.ncbi.nlm.nih.gov/pubmed/17623079 http://dx.doi.org/10.1186/1747-1028-2-19 |
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author | Torres-Rosell, Jordi De Piccoli, Giacomo Aragón, Luis |
author_facet | Torres-Rosell, Jordi De Piccoli, Giacomo Aragón, Luis |
author_sort | Torres-Rosell, Jordi |
collection | PubMed |
description | Completion of DNA replication before mitosis is essential for genome stability and cell viability. Cellular controls called checkpoints act as surveillance mechanisms capable of detecting errors and blocking cell cycle progression to allow time for those errors to be corrected. An important question in the cell cycle field is whether eukaryotic cells possess mechanisms that monitor ongoing DNA replication and make sure that all chromosomes are fully replicated before entering mitosis, that is whether a replication-completion checkpoint exists. From recent studies with smc5–smc6 mutants it appears that yeast cells can enter anaphase without noticing that replication in the ribosomal DNA array was unfinished. smc5–smc6 mutants are proficient in all known cellular checkpoints, namely the S phase checkpoint, DNA-damage checkpoint, and spindle checkpoint, thus suggesting that none of these checkpoints can monitor the presence of unreplicated segments or the unhindered progression of forks in rDNA. Therefore, these results strongly suggest that normal yeast cells do not contain a DNA replication-completion checkpoint. |
format | Text |
id | pubmed-1976610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-19766102007-09-15 Can eukaryotic cells monitor the presence of unreplicated DNA? Torres-Rosell, Jordi De Piccoli, Giacomo Aragón, Luis Cell Div Review Completion of DNA replication before mitosis is essential for genome stability and cell viability. Cellular controls called checkpoints act as surveillance mechanisms capable of detecting errors and blocking cell cycle progression to allow time for those errors to be corrected. An important question in the cell cycle field is whether eukaryotic cells possess mechanisms that monitor ongoing DNA replication and make sure that all chromosomes are fully replicated before entering mitosis, that is whether a replication-completion checkpoint exists. From recent studies with smc5–smc6 mutants it appears that yeast cells can enter anaphase without noticing that replication in the ribosomal DNA array was unfinished. smc5–smc6 mutants are proficient in all known cellular checkpoints, namely the S phase checkpoint, DNA-damage checkpoint, and spindle checkpoint, thus suggesting that none of these checkpoints can monitor the presence of unreplicated segments or the unhindered progression of forks in rDNA. Therefore, these results strongly suggest that normal yeast cells do not contain a DNA replication-completion checkpoint. BioMed Central 2007-07-10 /pmc/articles/PMC1976610/ /pubmed/17623079 http://dx.doi.org/10.1186/1747-1028-2-19 Text en Copyright © 2007 Torres-Rosell et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Torres-Rosell, Jordi De Piccoli, Giacomo Aragón, Luis Can eukaryotic cells monitor the presence of unreplicated DNA? |
title | Can eukaryotic cells monitor the presence of unreplicated DNA? |
title_full | Can eukaryotic cells monitor the presence of unreplicated DNA? |
title_fullStr | Can eukaryotic cells monitor the presence of unreplicated DNA? |
title_full_unstemmed | Can eukaryotic cells monitor the presence of unreplicated DNA? |
title_short | Can eukaryotic cells monitor the presence of unreplicated DNA? |
title_sort | can eukaryotic cells monitor the presence of unreplicated dna? |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976610/ https://www.ncbi.nlm.nih.gov/pubmed/17623079 http://dx.doi.org/10.1186/1747-1028-2-19 |
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