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Can eukaryotic cells monitor the presence of unreplicated DNA?

Completion of DNA replication before mitosis is essential for genome stability and cell viability. Cellular controls called checkpoints act as surveillance mechanisms capable of detecting errors and blocking cell cycle progression to allow time for those errors to be corrected. An important question...

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Detalles Bibliográficos
Autores principales: Torres-Rosell, Jordi, De Piccoli, Giacomo, Aragón, Luis
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976610/
https://www.ncbi.nlm.nih.gov/pubmed/17623079
http://dx.doi.org/10.1186/1747-1028-2-19
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author Torres-Rosell, Jordi
De Piccoli, Giacomo
Aragón, Luis
author_facet Torres-Rosell, Jordi
De Piccoli, Giacomo
Aragón, Luis
author_sort Torres-Rosell, Jordi
collection PubMed
description Completion of DNA replication before mitosis is essential for genome stability and cell viability. Cellular controls called checkpoints act as surveillance mechanisms capable of detecting errors and blocking cell cycle progression to allow time for those errors to be corrected. An important question in the cell cycle field is whether eukaryotic cells possess mechanisms that monitor ongoing DNA replication and make sure that all chromosomes are fully replicated before entering mitosis, that is whether a replication-completion checkpoint exists. From recent studies with smc5–smc6 mutants it appears that yeast cells can enter anaphase without noticing that replication in the ribosomal DNA array was unfinished. smc5–smc6 mutants are proficient in all known cellular checkpoints, namely the S phase checkpoint, DNA-damage checkpoint, and spindle checkpoint, thus suggesting that none of these checkpoints can monitor the presence of unreplicated segments or the unhindered progression of forks in rDNA. Therefore, these results strongly suggest that normal yeast cells do not contain a DNA replication-completion checkpoint.
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spelling pubmed-19766102007-09-15 Can eukaryotic cells monitor the presence of unreplicated DNA? Torres-Rosell, Jordi De Piccoli, Giacomo Aragón, Luis Cell Div Review Completion of DNA replication before mitosis is essential for genome stability and cell viability. Cellular controls called checkpoints act as surveillance mechanisms capable of detecting errors and blocking cell cycle progression to allow time for those errors to be corrected. An important question in the cell cycle field is whether eukaryotic cells possess mechanisms that monitor ongoing DNA replication and make sure that all chromosomes are fully replicated before entering mitosis, that is whether a replication-completion checkpoint exists. From recent studies with smc5–smc6 mutants it appears that yeast cells can enter anaphase without noticing that replication in the ribosomal DNA array was unfinished. smc5–smc6 mutants are proficient in all known cellular checkpoints, namely the S phase checkpoint, DNA-damage checkpoint, and spindle checkpoint, thus suggesting that none of these checkpoints can monitor the presence of unreplicated segments or the unhindered progression of forks in rDNA. Therefore, these results strongly suggest that normal yeast cells do not contain a DNA replication-completion checkpoint. BioMed Central 2007-07-10 /pmc/articles/PMC1976610/ /pubmed/17623079 http://dx.doi.org/10.1186/1747-1028-2-19 Text en Copyright © 2007 Torres-Rosell et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Torres-Rosell, Jordi
De Piccoli, Giacomo
Aragón, Luis
Can eukaryotic cells monitor the presence of unreplicated DNA?
title Can eukaryotic cells monitor the presence of unreplicated DNA?
title_full Can eukaryotic cells monitor the presence of unreplicated DNA?
title_fullStr Can eukaryotic cells monitor the presence of unreplicated DNA?
title_full_unstemmed Can eukaryotic cells monitor the presence of unreplicated DNA?
title_short Can eukaryotic cells monitor the presence of unreplicated DNA?
title_sort can eukaryotic cells monitor the presence of unreplicated dna?
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976610/
https://www.ncbi.nlm.nih.gov/pubmed/17623079
http://dx.doi.org/10.1186/1747-1028-2-19
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