Tumour and cellular localization by use of monoclonal and polyclonal antibodies to placental alkaline phosphatase.

Monoclonal and polyclonal antibodies against placental alkaline phosphatase (PLAP) were evaluated for tumour immunolocalization of human PLAP-producing Hep 2 tumours in nude mice. The antibodies were labelled with 125I and injected i.p. in mice with developing Hep 2 tumours. The distribution of 125I...

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Autores principales: Jeppsson, A., Wahren, B., Millán, J. L., Stigbrand, T.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1984
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976696/
https://www.ncbi.nlm.nih.gov/pubmed/6365130
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author Jeppsson, A.
Wahren, B.
Millán, J. L.
Stigbrand, T.
author_facet Jeppsson, A.
Wahren, B.
Millán, J. L.
Stigbrand, T.
author_sort Jeppsson, A.
collection PubMed
description Monoclonal and polyclonal antibodies against placental alkaline phosphatase (PLAP) were evaluated for tumour immunolocalization of human PLAP-producing Hep 2 tumours in nude mice. The antibodies were labelled with 125I and injected i.p. in mice with developing Hep 2 tumours. The distribution of 125I-anti PLAP in various tissues showed that the labelled antibody was enriched in the tumour, the mean concentration ratio being 7.1 and 6.8 for polyclonal and monoclonal antibodies, respectively. A PLAP negative tumour (RD) showed a mean ratio of 1.2. There was a positive correlation between PLAP content and uptake of labelled antibody in the tumours. Hep 2 tumour cells in tissue culture showed 100% positivity for PLAP, while imprints of the tumour after passage in nude mice showed 40-50% positivity. PLAP offers potential as a useful marker for localizing tumours in humans. IMAGES:
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spelling pubmed-19766962009-09-10 Tumour and cellular localization by use of monoclonal and polyclonal antibodies to placental alkaline phosphatase. Jeppsson, A. Wahren, B. Millán, J. L. Stigbrand, T. Br J Cancer Research Article Monoclonal and polyclonal antibodies against placental alkaline phosphatase (PLAP) were evaluated for tumour immunolocalization of human PLAP-producing Hep 2 tumours in nude mice. The antibodies were labelled with 125I and injected i.p. in mice with developing Hep 2 tumours. The distribution of 125I-anti PLAP in various tissues showed that the labelled antibody was enriched in the tumour, the mean concentration ratio being 7.1 and 6.8 for polyclonal and monoclonal antibodies, respectively. A PLAP negative tumour (RD) showed a mean ratio of 1.2. There was a positive correlation between PLAP content and uptake of labelled antibody in the tumours. Hep 2 tumour cells in tissue culture showed 100% positivity for PLAP, while imprints of the tumour after passage in nude mice showed 40-50% positivity. PLAP offers potential as a useful marker for localizing tumours in humans. IMAGES: Nature Publishing Group 1984-02 /pmc/articles/PMC1976696/ /pubmed/6365130 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Jeppsson, A.
Wahren, B.
Millán, J. L.
Stigbrand, T.
Tumour and cellular localization by use of monoclonal and polyclonal antibodies to placental alkaline phosphatase.
title Tumour and cellular localization by use of monoclonal and polyclonal antibodies to placental alkaline phosphatase.
title_full Tumour and cellular localization by use of monoclonal and polyclonal antibodies to placental alkaline phosphatase.
title_fullStr Tumour and cellular localization by use of monoclonal and polyclonal antibodies to placental alkaline phosphatase.
title_full_unstemmed Tumour and cellular localization by use of monoclonal and polyclonal antibodies to placental alkaline phosphatase.
title_short Tumour and cellular localization by use of monoclonal and polyclonal antibodies to placental alkaline phosphatase.
title_sort tumour and cellular localization by use of monoclonal and polyclonal antibodies to placental alkaline phosphatase.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976696/
https://www.ncbi.nlm.nih.gov/pubmed/6365130
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