Improved optical detection of colony enlargement and drug cytotoxicity in primary soft agar cultures of human solid tumour cells.

The presence of cellular aggregates in cell suspensions derived from human solid tumours often complicates subsequent evaluation of colony formation in primary soft agar cultures (Agrez et al., 1982b). In the present study, performance of a conventional colony formation assay was observed to lack su...

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Autores principales: Alley, M. C., Lieber, M. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1984
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976703/
https://www.ncbi.nlm.nih.gov/pubmed/6199034
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author Alley, M. C.
Lieber, M. M.
author_facet Alley, M. C.
Lieber, M. M.
author_sort Alley, M. C.
collection PubMed
description The presence of cellular aggregates in cell suspensions derived from human solid tumours often complicates subsequent evaluation of colony formation in primary soft agar cultures (Agrez et al., 1982b). In the present study, performance of a conventional colony formation assay was observed to lack sufficient sensitivity to identify growth and active chemotherapeutic agents in the majority of specimen cultures. Modification of conventional methodologies to include filtration of cell suspensions, use of "proliferation control" and "cytotoxicity control" cultures as well as vital staining were found to be essential for the valid assessment of primary soft agar cultures in our laboratory. In addition, application of drugs to culture surface in place of culture incorporation appeared to facilitate culture performance and drug sensitivity testing. IMAGES:
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spelling pubmed-19767032009-09-10 Improved optical detection of colony enlargement and drug cytotoxicity in primary soft agar cultures of human solid tumour cells. Alley, M. C. Lieber, M. M. Br J Cancer Research Article The presence of cellular aggregates in cell suspensions derived from human solid tumours often complicates subsequent evaluation of colony formation in primary soft agar cultures (Agrez et al., 1982b). In the present study, performance of a conventional colony formation assay was observed to lack sufficient sensitivity to identify growth and active chemotherapeutic agents in the majority of specimen cultures. Modification of conventional methodologies to include filtration of cell suspensions, use of "proliferation control" and "cytotoxicity control" cultures as well as vital staining were found to be essential for the valid assessment of primary soft agar cultures in our laboratory. In addition, application of drugs to culture surface in place of culture incorporation appeared to facilitate culture performance and drug sensitivity testing. IMAGES: Nature Publishing Group 1984-02 /pmc/articles/PMC1976703/ /pubmed/6199034 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Alley, M. C.
Lieber, M. M.
Improved optical detection of colony enlargement and drug cytotoxicity in primary soft agar cultures of human solid tumour cells.
title Improved optical detection of colony enlargement and drug cytotoxicity in primary soft agar cultures of human solid tumour cells.
title_full Improved optical detection of colony enlargement and drug cytotoxicity in primary soft agar cultures of human solid tumour cells.
title_fullStr Improved optical detection of colony enlargement and drug cytotoxicity in primary soft agar cultures of human solid tumour cells.
title_full_unstemmed Improved optical detection of colony enlargement and drug cytotoxicity in primary soft agar cultures of human solid tumour cells.
title_short Improved optical detection of colony enlargement and drug cytotoxicity in primary soft agar cultures of human solid tumour cells.
title_sort improved optical detection of colony enlargement and drug cytotoxicity in primary soft agar cultures of human solid tumour cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976703/
https://www.ncbi.nlm.nih.gov/pubmed/6199034
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