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Cellular DNA content--a stable feature in epithelial ovarian cancer.

Detailed flow cytometric analysis of cellular DNA content was performed on neoplastic tissue from 33 patients with malignant common epithelial ovarian tumours in order to investigate the intratumoral stability of ploidy and proliferative fraction. There was a remarkable stability, both spatial and t...

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Autores principales: Friedlander, M. L., Taylor, I. W., Russell, P., Tattersall, M. H.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1984
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976707/
https://www.ncbi.nlm.nih.gov/pubmed/6696818
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author Friedlander, M. L.
Taylor, I. W.
Russell, P.
Tattersall, M. H.
author_facet Friedlander, M. L.
Taylor, I. W.
Russell, P.
Tattersall, M. H.
author_sort Friedlander, M. L.
collection PubMed
description Detailed flow cytometric analysis of cellular DNA content was performed on neoplastic tissue from 33 patients with malignant common epithelial ovarian tumours in order to investigate the intratumoral stability of ploidy and proliferative fraction. There was a remarkable stability, both spatial and temporal, in the DNA pattern for any particular tumour. Of 24 tumours that were analysed in multiple areas tumour ploidy was found to be a stable marker in all but 3 cases where regional variations were evident. In 9 patients serial analyses were performed on tumour obtained either at initial diagnosis (6 patients) or second look laparotomy (3 patients) and then some time later (7-17 months) at relapse or death and in all cases the tumour ploidy remained unchanged. In addition, 10 ovarian carcinomas established in nude mice have maintained a DNA content during serial passage similar to that of the original implanted tumour. In contrast in 50% of tumours that were evaluable for S-phase analysis we demonstrated a considerable intratumoral variability in the S-phase fraction. We conclude that cellular DNA content is a stable feature of ovarian carcinoma while S-phase fraction is commonly subject to intratumoral variation.
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spelling pubmed-19767072009-09-10 Cellular DNA content--a stable feature in epithelial ovarian cancer. Friedlander, M. L. Taylor, I. W. Russell, P. Tattersall, M. H. Br J Cancer Research Article Detailed flow cytometric analysis of cellular DNA content was performed on neoplastic tissue from 33 patients with malignant common epithelial ovarian tumours in order to investigate the intratumoral stability of ploidy and proliferative fraction. There was a remarkable stability, both spatial and temporal, in the DNA pattern for any particular tumour. Of 24 tumours that were analysed in multiple areas tumour ploidy was found to be a stable marker in all but 3 cases where regional variations were evident. In 9 patients serial analyses were performed on tumour obtained either at initial diagnosis (6 patients) or second look laparotomy (3 patients) and then some time later (7-17 months) at relapse or death and in all cases the tumour ploidy remained unchanged. In addition, 10 ovarian carcinomas established in nude mice have maintained a DNA content during serial passage similar to that of the original implanted tumour. In contrast in 50% of tumours that were evaluable for S-phase analysis we demonstrated a considerable intratumoral variability in the S-phase fraction. We conclude that cellular DNA content is a stable feature of ovarian carcinoma while S-phase fraction is commonly subject to intratumoral variation. Nature Publishing Group 1984-02 /pmc/articles/PMC1976707/ /pubmed/6696818 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Friedlander, M. L.
Taylor, I. W.
Russell, P.
Tattersall, M. H.
Cellular DNA content--a stable feature in epithelial ovarian cancer.
title Cellular DNA content--a stable feature in epithelial ovarian cancer.
title_full Cellular DNA content--a stable feature in epithelial ovarian cancer.
title_fullStr Cellular DNA content--a stable feature in epithelial ovarian cancer.
title_full_unstemmed Cellular DNA content--a stable feature in epithelial ovarian cancer.
title_short Cellular DNA content--a stable feature in epithelial ovarian cancer.
title_sort cellular dna content--a stable feature in epithelial ovarian cancer.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976707/
https://www.ncbi.nlm.nih.gov/pubmed/6696818
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