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The cellular specificity of haemopoietic stem cell proliferation regulators.

The range of specificity of the CFU-S proliferation inhibitor and stimulator which are produced endogenously in the bone marrow has been investigated by measuring their effects on the proportion of cells killed by tritiated thymidine in mixed colony- (CFC-mix), erythroid burst- (BFU-E) and granulocy...

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Autores principales: Tejero, C., Testa, N. G., Lord, B. I.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1984
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976792/
https://www.ncbi.nlm.nih.gov/pubmed/6331841
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author Tejero, C.
Testa, N. G.
Lord, B. I.
author_facet Tejero, C.
Testa, N. G.
Lord, B. I.
author_sort Tejero, C.
collection PubMed
description The range of specificity of the CFU-S proliferation inhibitor and stimulator which are produced endogenously in the bone marrow has been investigated by measuring their effects on the proportion of cells killed by tritiated thymidine in mixed colony- (CFC-mix), erythroid burst- (BFU-E) and granulocyte/macrophage colony- (GM-CFC) forming cells as well as spleen colony forming units (CFU-S). Both CFU-S and CFC-mix were triggered by the stimulator into DNA-synthesis but BFU-E and GM-CFC were unaffected. The range of activity of the inhibitor was confined solely to the CFU-S population. This defined the specificity of both inhibitor and stimulator for the multipotent cells. The differential sensitivity of CFU-S and CFC-mix to the inhibitor and the lack of it for the stimulator suggested (a) that the CFC-mix is a relatively mature subpopulation of the CFU-S compartment and (b) that the relative sensitivity of a CFU-S to these factors changes as it matures from the early stem cell stage (Inhibitor-sensitive) to the more mature stages (Stimulator-sensitive) before becoming committed to a specific line of differentiation. The specificity of the inhibitor for haemopoietic stem cells suggests its potential value during chemotherapeutic procedures.
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spelling pubmed-19767922009-09-10 The cellular specificity of haemopoietic stem cell proliferation regulators. Tejero, C. Testa, N. G. Lord, B. I. Br J Cancer Research Article The range of specificity of the CFU-S proliferation inhibitor and stimulator which are produced endogenously in the bone marrow has been investigated by measuring their effects on the proportion of cells killed by tritiated thymidine in mixed colony- (CFC-mix), erythroid burst- (BFU-E) and granulocyte/macrophage colony- (GM-CFC) forming cells as well as spleen colony forming units (CFU-S). Both CFU-S and CFC-mix were triggered by the stimulator into DNA-synthesis but BFU-E and GM-CFC were unaffected. The range of activity of the inhibitor was confined solely to the CFU-S population. This defined the specificity of both inhibitor and stimulator for the multipotent cells. The differential sensitivity of CFU-S and CFC-mix to the inhibitor and the lack of it for the stimulator suggested (a) that the CFC-mix is a relatively mature subpopulation of the CFU-S compartment and (b) that the relative sensitivity of a CFU-S to these factors changes as it matures from the early stem cell stage (Inhibitor-sensitive) to the more mature stages (Stimulator-sensitive) before becoming committed to a specific line of differentiation. The specificity of the inhibitor for haemopoietic stem cells suggests its potential value during chemotherapeutic procedures. Nature Publishing Group 1984-09 /pmc/articles/PMC1976792/ /pubmed/6331841 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Tejero, C.
Testa, N. G.
Lord, B. I.
The cellular specificity of haemopoietic stem cell proliferation regulators.
title The cellular specificity of haemopoietic stem cell proliferation regulators.
title_full The cellular specificity of haemopoietic stem cell proliferation regulators.
title_fullStr The cellular specificity of haemopoietic stem cell proliferation regulators.
title_full_unstemmed The cellular specificity of haemopoietic stem cell proliferation regulators.
title_short The cellular specificity of haemopoietic stem cell proliferation regulators.
title_sort cellular specificity of haemopoietic stem cell proliferation regulators.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976792/
https://www.ncbi.nlm.nih.gov/pubmed/6331841
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