Relationship between cell ploidy and glucocorticoid induced death in human lymphoid cell lines.

We have found a relationship between sensitivity to glucocorticoid induced cell death (at 10 microM glucocorticoid) and ploidy in the human lymphoid cell line CCRF/CEM-C7. Most sensitive clones are diploid, whilst resistant clones and the resistant parent line CCRF/CEM are tetraploid. Diploid sensit...

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Autores principales: Dyson, J. E., Quirke, P., Bird, C. C., McLaughlin, J. B., Surrey, C. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1984
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976844/
https://www.ncbi.nlm.nih.gov/pubmed/6203547
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author Dyson, J. E.
Quirke, P.
Bird, C. C.
McLaughlin, J. B.
Surrey, C. R.
author_facet Dyson, J. E.
Quirke, P.
Bird, C. C.
McLaughlin, J. B.
Surrey, C. R.
author_sort Dyson, J. E.
collection PubMed
description We have found a relationship between sensitivity to glucocorticoid induced cell death (at 10 microM glucocorticoid) and ploidy in the human lymphoid cell line CCRF/CEM-C7. Most sensitive clones are diploid, whilst resistant clones and the resistant parent line CCRF/CEM are tetraploid. Diploid sensitive clones have a tendency to become aneuploid within a few months of isolation, with alterations in their kinetic responses to glucocorticoids. This is followed by a doubling in DNA content which results in reversion to the tetraploid glucocorticoid resistant state of the parent line CCRF/CEM. A few sensitive clones have been found to be tetraploid but with different kinetic responses to glucocorticoids as compared to diploid clones. The principal difference being an extended lag period (48-72 h) prior to lethal response. The relationship between ploidy and glucocorticoid sensitivity does not appear to extend to other human lymphoid cell lines. IMAGES:
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spelling pubmed-19768442009-09-10 Relationship between cell ploidy and glucocorticoid induced death in human lymphoid cell lines. Dyson, J. E. Quirke, P. Bird, C. C. McLaughlin, J. B. Surrey, C. R. Br J Cancer Research Article We have found a relationship between sensitivity to glucocorticoid induced cell death (at 10 microM glucocorticoid) and ploidy in the human lymphoid cell line CCRF/CEM-C7. Most sensitive clones are diploid, whilst resistant clones and the resistant parent line CCRF/CEM are tetraploid. Diploid sensitive clones have a tendency to become aneuploid within a few months of isolation, with alterations in their kinetic responses to glucocorticoids. This is followed by a doubling in DNA content which results in reversion to the tetraploid glucocorticoid resistant state of the parent line CCRF/CEM. A few sensitive clones have been found to be tetraploid but with different kinetic responses to glucocorticoids as compared to diploid clones. The principal difference being an extended lag period (48-72 h) prior to lethal response. The relationship between ploidy and glucocorticoid sensitivity does not appear to extend to other human lymphoid cell lines. IMAGES: Nature Publishing Group 1984-06 /pmc/articles/PMC1976844/ /pubmed/6203547 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Dyson, J. E.
Quirke, P.
Bird, C. C.
McLaughlin, J. B.
Surrey, C. R.
Relationship between cell ploidy and glucocorticoid induced death in human lymphoid cell lines.
title Relationship between cell ploidy and glucocorticoid induced death in human lymphoid cell lines.
title_full Relationship between cell ploidy and glucocorticoid induced death in human lymphoid cell lines.
title_fullStr Relationship between cell ploidy and glucocorticoid induced death in human lymphoid cell lines.
title_full_unstemmed Relationship between cell ploidy and glucocorticoid induced death in human lymphoid cell lines.
title_short Relationship between cell ploidy and glucocorticoid induced death in human lymphoid cell lines.
title_sort relationship between cell ploidy and glucocorticoid induced death in human lymphoid cell lines.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976844/
https://www.ncbi.nlm.nih.gov/pubmed/6203547
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