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The response of tumour cells to radiation and cytotoxic drugs--a comparison of clonogenic and isotope uptake assays.

We have carried out a series of experiments to compare the response to radiation and drugs of cells disaggregated from solid tumours as assayed by clonogenic survival and by an isotope incorporation method. This latter assay consisted of measuring the 24 h uptake of tritium labelled thymidine into c...

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Autores principales: Twentyman, P. R., Walls, G. A., Wright, K. A.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1984
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976983/
https://www.ncbi.nlm.nih.gov/pubmed/6093839
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author Twentyman, P. R.
Walls, G. A.
Wright, K. A.
author_facet Twentyman, P. R.
Walls, G. A.
Wright, K. A.
author_sort Twentyman, P. R.
collection PubMed
description We have carried out a series of experiments to compare the response to radiation and drugs of cells disaggregated from solid tumours as assayed by clonogenic survival and by an isotope incorporation method. This latter assay consisted of measuring the 24 h uptake of tritium labelled thymidine into cells plated in liquid medium upon a layer of semi-solid agar. The isotope was administered 4 days after plating. For cells from the RIF-1 mouse tumour, good agreement was seen between response to radiation, adriamycin, vincristine and CCNU as measured by the two assays. The two curves for radiation response, for example, showed similar shoulders and subsequent exponential regions. For cells from xenografts of the NCI-H69 human small cell lung cancer line, the response to radiation was dose-related for both assays, but the curve for clonogenic assay was about twice as steep as that for isotope uptake. For a range of five cytotoxic drugs, good agreement was seen between the two assays over the first 1 1/2 decades of response but with a tendency for the isotope uptake curve to plateau with further increasing drug dose. It appears that, at least for these two well-defined experimental tumour systems, the isotope uptake assay can provide a rapid quantitative assessment of cellular drug and radiation sensitivity comparable to that provided by clonogenic assay but in a much shorter period of time.
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spelling pubmed-19769832009-09-10 The response of tumour cells to radiation and cytotoxic drugs--a comparison of clonogenic and isotope uptake assays. Twentyman, P. R. Walls, G. A. Wright, K. A. Br J Cancer Research Article We have carried out a series of experiments to compare the response to radiation and drugs of cells disaggregated from solid tumours as assayed by clonogenic survival and by an isotope incorporation method. This latter assay consisted of measuring the 24 h uptake of tritium labelled thymidine into cells plated in liquid medium upon a layer of semi-solid agar. The isotope was administered 4 days after plating. For cells from the RIF-1 mouse tumour, good agreement was seen between response to radiation, adriamycin, vincristine and CCNU as measured by the two assays. The two curves for radiation response, for example, showed similar shoulders and subsequent exponential regions. For cells from xenografts of the NCI-H69 human small cell lung cancer line, the response to radiation was dose-related for both assays, but the curve for clonogenic assay was about twice as steep as that for isotope uptake. For a range of five cytotoxic drugs, good agreement was seen between the two assays over the first 1 1/2 decades of response but with a tendency for the isotope uptake curve to plateau with further increasing drug dose. It appears that, at least for these two well-defined experimental tumour systems, the isotope uptake assay can provide a rapid quantitative assessment of cellular drug and radiation sensitivity comparable to that provided by clonogenic assay but in a much shorter period of time. Nature Publishing Group 1984-11 /pmc/articles/PMC1976983/ /pubmed/6093839 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Twentyman, P. R.
Walls, G. A.
Wright, K. A.
The response of tumour cells to radiation and cytotoxic drugs--a comparison of clonogenic and isotope uptake assays.
title The response of tumour cells to radiation and cytotoxic drugs--a comparison of clonogenic and isotope uptake assays.
title_full The response of tumour cells to radiation and cytotoxic drugs--a comparison of clonogenic and isotope uptake assays.
title_fullStr The response of tumour cells to radiation and cytotoxic drugs--a comparison of clonogenic and isotope uptake assays.
title_full_unstemmed The response of tumour cells to radiation and cytotoxic drugs--a comparison of clonogenic and isotope uptake assays.
title_short The response of tumour cells to radiation and cytotoxic drugs--a comparison of clonogenic and isotope uptake assays.
title_sort response of tumour cells to radiation and cytotoxic drugs--a comparison of clonogenic and isotope uptake assays.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976983/
https://www.ncbi.nlm.nih.gov/pubmed/6093839
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