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The response to cytotoxic drugs of EMT6 cells treated either as intact or disaggregated spheroids.

We have compared the response to a number of cytotoxic drugs of cells treated either within intact multicellular spheroids or as isolated cells following spheroid disaggregation. The cells used were of the EMT6/Ca/VJAC mouse tumour line and spheroids were treated or disaggregated at a mean diameter...

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Detalles Bibliográficos
Autores principales: Kwok, T. T., Twentyman, P. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1985
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977032/
https://www.ncbi.nlm.nih.gov/pubmed/3966978
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author Kwok, T. T.
Twentyman, P. R.
author_facet Kwok, T. T.
Twentyman, P. R.
author_sort Kwok, T. T.
collection PubMed
description We have compared the response to a number of cytotoxic drugs of cells treated either within intact multicellular spheroids or as isolated cells following spheroid disaggregation. The cells used were of the EMT6/Ca/VJAC mouse tumour line and spheroids were treated or disaggregated at a mean diameter of 250 micron. The response of cell to nitrogen mustard (HN2) or CCNU was similar under the two exposure conditions and we conclude that factors related to spheroid structure (i.e. drug penetrability, intercellular contact effect and microenvironment within the spheroid) do not influence the initial response to these agents. Recovery of potentially lethal damage occurring over 24 h, however, greatly modifies the level of cell killing in intact spheroids. EMT6 cells were found to be extremely resistant to vincristine under all exposure conditions. For adriamycin (ADM), cells were always initially more sensitive when exposed to the drug in suspension rather than in intact spheroids. When ADM exposure was prolonged beyond 1 h, however, delaying spheroid disaggregation for 24 h led to increased cell kill and reduced differential between the two conditions of exposure. The data suggest that both drug penetration problems and other factors related to spheroid structure are involved in determining the response of cells in small spheroids to ADM.
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spelling pubmed-19770322009-09-10 The response to cytotoxic drugs of EMT6 cells treated either as intact or disaggregated spheroids. Kwok, T. T. Twentyman, P. R. Br J Cancer Research Article We have compared the response to a number of cytotoxic drugs of cells treated either within intact multicellular spheroids or as isolated cells following spheroid disaggregation. The cells used were of the EMT6/Ca/VJAC mouse tumour line and spheroids were treated or disaggregated at a mean diameter of 250 micron. The response of cell to nitrogen mustard (HN2) or CCNU was similar under the two exposure conditions and we conclude that factors related to spheroid structure (i.e. drug penetrability, intercellular contact effect and microenvironment within the spheroid) do not influence the initial response to these agents. Recovery of potentially lethal damage occurring over 24 h, however, greatly modifies the level of cell killing in intact spheroids. EMT6 cells were found to be extremely resistant to vincristine under all exposure conditions. For adriamycin (ADM), cells were always initially more sensitive when exposed to the drug in suspension rather than in intact spheroids. When ADM exposure was prolonged beyond 1 h, however, delaying spheroid disaggregation for 24 h led to increased cell kill and reduced differential between the two conditions of exposure. The data suggest that both drug penetration problems and other factors related to spheroid structure are involved in determining the response of cells in small spheroids to ADM. Nature Publishing Group 1985-02 /pmc/articles/PMC1977032/ /pubmed/3966978 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Kwok, T. T.
Twentyman, P. R.
The response to cytotoxic drugs of EMT6 cells treated either as intact or disaggregated spheroids.
title The response to cytotoxic drugs of EMT6 cells treated either as intact or disaggregated spheroids.
title_full The response to cytotoxic drugs of EMT6 cells treated either as intact or disaggregated spheroids.
title_fullStr The response to cytotoxic drugs of EMT6 cells treated either as intact or disaggregated spheroids.
title_full_unstemmed The response to cytotoxic drugs of EMT6 cells treated either as intact or disaggregated spheroids.
title_short The response to cytotoxic drugs of EMT6 cells treated either as intact or disaggregated spheroids.
title_sort response to cytotoxic drugs of emt6 cells treated either as intact or disaggregated spheroids.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977032/
https://www.ncbi.nlm.nih.gov/pubmed/3966978
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