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Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours.
A monoclonal antibody (H17E2) recognising both placental alkaline phosphatase (PLAP) and testicular PLAP-like alkaline phosphatase was incorporated in a solid phase immunoassay. This was used to measure levels of PLAP in 257 sera from 148 patients with germ cell neoplasms of the testis. High levels...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1985
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977048/ https://www.ncbi.nlm.nih.gov/pubmed/3994908 |
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author | Epenetos, A. A. Munro, A. J. Tucker, D. F. Gregory, W. Duncan, W. MacDougall, R. H. Faux, M. Travers, P. Bodmer, W. F. |
author_facet | Epenetos, A. A. Munro, A. J. Tucker, D. F. Gregory, W. Duncan, W. MacDougall, R. H. Faux, M. Travers, P. Bodmer, W. F. |
author_sort | Epenetos, A. A. |
collection | PubMed |
description | A monoclonal antibody (H17E2) recognising both placental alkaline phosphatase (PLAP) and testicular PLAP-like alkaline phosphatase was incorporated in a solid phase immunoassay. This was used to measure levels of PLAP in 257 sera from 148 patients with germ cell neoplasms of the testis. High levels of PLAP were found in all patients with active seminomas (mean 0.85 O.D.) compared to those in clinical remission (mean 0.20 O.D.) (P less than 0.0001). More importantly, changing levels of PLAP correlated with the course of disease in 79 samples from 33 patients with seminoma (P less than 0.0001). Elevated PLAP levels were also noted in patients in remission who were smokers (mean 0.32 O.D.) compared to non-smokers (mean 0.15 O.D.) (P less than 0.001). These data demonstrate that determination of PLAP levels using this sensitive immunoassay is an important new adjunct in the monitoring of the response to treatment in patients with seminoma. |
format | Text |
id | pubmed-1977048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1985 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-19770482009-09-10 Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours. Epenetos, A. A. Munro, A. J. Tucker, D. F. Gregory, W. Duncan, W. MacDougall, R. H. Faux, M. Travers, P. Bodmer, W. F. Br J Cancer Research Article A monoclonal antibody (H17E2) recognising both placental alkaline phosphatase (PLAP) and testicular PLAP-like alkaline phosphatase was incorporated in a solid phase immunoassay. This was used to measure levels of PLAP in 257 sera from 148 patients with germ cell neoplasms of the testis. High levels of PLAP were found in all patients with active seminomas (mean 0.85 O.D.) compared to those in clinical remission (mean 0.20 O.D.) (P less than 0.0001). More importantly, changing levels of PLAP correlated with the course of disease in 79 samples from 33 patients with seminoma (P less than 0.0001). Elevated PLAP levels were also noted in patients in remission who were smokers (mean 0.32 O.D.) compared to non-smokers (mean 0.15 O.D.) (P less than 0.001). These data demonstrate that determination of PLAP levels using this sensitive immunoassay is an important new adjunct in the monitoring of the response to treatment in patients with seminoma. Nature Publishing Group 1985-05 /pmc/articles/PMC1977048/ /pubmed/3994908 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Epenetos, A. A. Munro, A. J. Tucker, D. F. Gregory, W. Duncan, W. MacDougall, R. H. Faux, M. Travers, P. Bodmer, W. F. Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours. |
title | Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours. |
title_full | Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours. |
title_fullStr | Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours. |
title_full_unstemmed | Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours. |
title_short | Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours. |
title_sort | monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977048/ https://www.ncbi.nlm.nih.gov/pubmed/3994908 |
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