A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2.

Two polyclonal rabbit antibodies to epithelial membrane antigen (EMA), two mouse monoclonal antibodies (E29 and HMFG-2), and a "cocktail" of these two monoclonals have been compared using an indirect immunoperoxidase technique. Sections from 25 tissues (17 malignant and 8 benign), were exa...

Descripción completa

Detalles Bibliográficos
Autores principales: Heyderman, E., Strudley, I., Powell, G., Richardson, T. C., Cordell, J. L., Mason, D. Y.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1985
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977186/
https://www.ncbi.nlm.nih.gov/pubmed/3899156
_version_ 1782135208305229824
author Heyderman, E.
Strudley, I.
Powell, G.
Richardson, T. C.
Cordell, J. L.
Mason, D. Y.
author_facet Heyderman, E.
Strudley, I.
Powell, G.
Richardson, T. C.
Cordell, J. L.
Mason, D. Y.
author_sort Heyderman, E.
collection PubMed
description Two polyclonal rabbit antibodies to epithelial membrane antigen (EMA), two mouse monoclonal antibodies (E29 and HMFG-2), and a "cocktail" of these two monoclonals have been compared using an indirect immunoperoxidase technique. Sections from 25 tissues (17 malignant and 8 benign), were examined. The distribution of staining with each of these reagents was similar, but the polyclonal antibodies produced stronger staining in colorectal carcinomas and lactating breast, whereas staining with the monoclonal antibodies was stronger in non-neoplastic pleural mesothelium and in pulmonary alveolar cells. When the two monoclonals were mixed there was no increase in staining intensity. E29 gave a "cleaner" result than HMFG-2, with better discrimination between cells and stroma, and is highly suitable for routine diagnostic histopathology. IMAGES:
format Text
id pubmed-1977186
institution National Center for Biotechnology Information
language English
publishDate 1985
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-19771862009-09-10 A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2. Heyderman, E. Strudley, I. Powell, G. Richardson, T. C. Cordell, J. L. Mason, D. Y. Br J Cancer Research Article Two polyclonal rabbit antibodies to epithelial membrane antigen (EMA), two mouse monoclonal antibodies (E29 and HMFG-2), and a "cocktail" of these two monoclonals have been compared using an indirect immunoperoxidase technique. Sections from 25 tissues (17 malignant and 8 benign), were examined. The distribution of staining with each of these reagents was similar, but the polyclonal antibodies produced stronger staining in colorectal carcinomas and lactating breast, whereas staining with the monoclonal antibodies was stronger in non-neoplastic pleural mesothelium and in pulmonary alveolar cells. When the two monoclonals were mixed there was no increase in staining intensity. E29 gave a "cleaner" result than HMFG-2, with better discrimination between cells and stroma, and is highly suitable for routine diagnostic histopathology. IMAGES: Nature Publishing Group 1985-09 /pmc/articles/PMC1977186/ /pubmed/3899156 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Heyderman, E.
Strudley, I.
Powell, G.
Richardson, T. C.
Cordell, J. L.
Mason, D. Y.
A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2.
title A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2.
title_full A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2.
title_fullStr A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2.
title_full_unstemmed A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2.
title_short A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2.
title_sort new monoclonal antibody to epithelial membrane antigen (ema)-e29. a comparison of its immunocytochemical reactivity with polyclonal anti-ema antibodies and with another monoclonal antibody, hmfg-2.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977186/
https://www.ncbi.nlm.nih.gov/pubmed/3899156
work_keys_str_mv AT heydermane anewmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2
AT strudleyi anewmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2
AT powellg anewmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2
AT richardsontc anewmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2
AT cordelljl anewmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2
AT masondy anewmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2
AT heydermane newmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2
AT strudleyi newmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2
AT powellg newmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2
AT richardsontc newmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2
AT cordelljl newmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2
AT masondy newmonoclonalantibodytoepithelialmembraneantigenemae29acomparisonofitsimmunocytochemicalreactivitywithpolyclonalantiemaantibodiesandwithanothermonoclonalantibodyhmfg2

Ejemplares similares