Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.

Two monoclonal murine antibodies have been raised against a delipidated extract of human cream. These antibodies were detected by immunohistological screening of hybridoma culture supernatants on sections of human breast tissue. One of those antibodies (E29) was subsequently screened against a range...

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Detalles Bibliográficos
Autores principales: Cordell, J., Richardson, T. C., Pulford, K. A., Ghosh, A. K., Gatter, K. C., Heyderman, E., Mason, D. Y.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1985
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977193/
https://www.ncbi.nlm.nih.gov/pubmed/3899155
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author Cordell, J.
Richardson, T. C.
Pulford, K. A.
Ghosh, A. K.
Gatter, K. C.
Heyderman, E.
Mason, D. Y.
author_facet Cordell, J.
Richardson, T. C.
Pulford, K. A.
Ghosh, A. K.
Gatter, K. C.
Heyderman, E.
Mason, D. Y.
author_sort Cordell, J.
collection PubMed
description Two monoclonal murine antibodies have been raised against a delipidated extract of human cream. These antibodies were detected by immunohistological screening of hybridoma culture supernatants on sections of human breast tissue. One of those antibodies (E29) was subsequently screened against a range of normal and neoplastic human tissues and shown to react with a wide variety of human epithelia and with mesothelial cells. Antibody E29 was unreactive with other cell types, with the exception of occasional plasma cells. Antibody E29 is suitable for use on paraffin embedded tissue and represents a valuable reagent for the identification of tumours of epithelial origin. IMAGES:
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spelling pubmed-19771932009-09-10 Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. Cordell, J. Richardson, T. C. Pulford, K. A. Ghosh, A. K. Gatter, K. C. Heyderman, E. Mason, D. Y. Br J Cancer Research Article Two monoclonal murine antibodies have been raised against a delipidated extract of human cream. These antibodies were detected by immunohistological screening of hybridoma culture supernatants on sections of human breast tissue. One of those antibodies (E29) was subsequently screened against a range of normal and neoplastic human tissues and shown to react with a wide variety of human epithelia and with mesothelial cells. Antibody E29 was unreactive with other cell types, with the exception of occasional plasma cells. Antibody E29 is suitable for use on paraffin embedded tissue and represents a valuable reagent for the identification of tumours of epithelial origin. IMAGES: Nature Publishing Group 1985-09 /pmc/articles/PMC1977193/ /pubmed/3899155 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Cordell, J.
Richardson, T. C.
Pulford, K. A.
Ghosh, A. K.
Gatter, K. C.
Heyderman, E.
Mason, D. Y.
Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.
title Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.
title_full Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.
title_fullStr Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.
title_full_unstemmed Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.
title_short Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.
title_sort production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977193/
https://www.ncbi.nlm.nih.gov/pubmed/3899155
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