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The generation of monoclonal antibodies against human pancreatic exocrine cancer: a study of six different immunisation regimes.

Six different immunisation regimes have been used to generate spleen cells with reactivity against human pancreatic exocrine cancer. Immunised spleen cells were fused with an NSO/1 myeloma line and supernatants from these hybridomas selectively screened for monoclonal antibodies which bound predomin...

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Autores principales: Grant, A. G., Harris, P. M., Heyderman, E., Larkin, S. E., Pym, B., Hermon-Taylor, J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1985
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977238/
https://www.ncbi.nlm.nih.gov/pubmed/4063133
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author Grant, A. G.
Harris, P. M.
Heyderman, E.
Larkin, S. E.
Pym, B.
Hermon-Taylor, J.
author_facet Grant, A. G.
Harris, P. M.
Heyderman, E.
Larkin, S. E.
Pym, B.
Hermon-Taylor, J.
author_sort Grant, A. G.
collection PubMed
description Six different immunisation regimes have been used to generate spleen cells with reactivity against human pancreatic exocrine cancer. Immunised spleen cells were fused with an NSO/1 myeloma line and supernatants from these hybridomas selectively screened for monoclonal antibodies which bound predominantly to a pancreatic cancer cell line (GER). The spleen cells from hairy litter mates immunised with pancreatic cancer xenograft homogenates and viable GER cells generated 13% of hybridoma supernatants which showed some selectivity for GER pancreatic cancer cells in a fixed cell ELISA assay. The other methods produced only 4% of hybrids with selectivity for GER cells. The antigen distribution on gluteraldehyde fixed cells was similar to that found for viable cell monolayers but many antigens were unstable on formalin fixation. Immunohistochemical staining of GER cells grown on glass slides showed a heterogeneity of antigen distribution with up to 70% of the cells exhibiting a vesicular pattern of staining. Fifty percent of the antibodies which bound to GER cells were also reactive against antigens in formalin-fixed paraffin-embedded tissue sections of the original GER tumour. Monoclonal antibody DD9E7 identified an antigen expressed on 12/14 pancreatic adenocarcinomas. The antibody showed strong staining of malignant luminal membranes and cytoplasm. The antigen was also present in normal salivary and sweat glands, and colon and breast carcinomas, but its tissue distribution was unlike that of CEA or EMA. The expression of this antigen in 12/14 of pancreatic carcinomas suggests that DD9E7 may be a useful reagent for pancreatic tumour detection. IMAGES:
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spelling pubmed-19772382009-09-10 The generation of monoclonal antibodies against human pancreatic exocrine cancer: a study of six different immunisation regimes. Grant, A. G. Harris, P. M. Heyderman, E. Larkin, S. E. Pym, B. Hermon-Taylor, J. Br J Cancer Research Article Six different immunisation regimes have been used to generate spleen cells with reactivity against human pancreatic exocrine cancer. Immunised spleen cells were fused with an NSO/1 myeloma line and supernatants from these hybridomas selectively screened for monoclonal antibodies which bound predominantly to a pancreatic cancer cell line (GER). The spleen cells from hairy litter mates immunised with pancreatic cancer xenograft homogenates and viable GER cells generated 13% of hybridoma supernatants which showed some selectivity for GER pancreatic cancer cells in a fixed cell ELISA assay. The other methods produced only 4% of hybrids with selectivity for GER cells. The antigen distribution on gluteraldehyde fixed cells was similar to that found for viable cell monolayers but many antigens were unstable on formalin fixation. Immunohistochemical staining of GER cells grown on glass slides showed a heterogeneity of antigen distribution with up to 70% of the cells exhibiting a vesicular pattern of staining. Fifty percent of the antibodies which bound to GER cells were also reactive against antigens in formalin-fixed paraffin-embedded tissue sections of the original GER tumour. Monoclonal antibody DD9E7 identified an antigen expressed on 12/14 pancreatic adenocarcinomas. The antibody showed strong staining of malignant luminal membranes and cytoplasm. The antigen was also present in normal salivary and sweat glands, and colon and breast carcinomas, but its tissue distribution was unlike that of CEA or EMA. The expression of this antigen in 12/14 of pancreatic carcinomas suggests that DD9E7 may be a useful reagent for pancreatic tumour detection. IMAGES: Nature Publishing Group 1985-10 /pmc/articles/PMC1977238/ /pubmed/4063133 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Grant, A. G.
Harris, P. M.
Heyderman, E.
Larkin, S. E.
Pym, B.
Hermon-Taylor, J.
The generation of monoclonal antibodies against human pancreatic exocrine cancer: a study of six different immunisation regimes.
title The generation of monoclonal antibodies against human pancreatic exocrine cancer: a study of six different immunisation regimes.
title_full The generation of monoclonal antibodies against human pancreatic exocrine cancer: a study of six different immunisation regimes.
title_fullStr The generation of monoclonal antibodies against human pancreatic exocrine cancer: a study of six different immunisation regimes.
title_full_unstemmed The generation of monoclonal antibodies against human pancreatic exocrine cancer: a study of six different immunisation regimes.
title_short The generation of monoclonal antibodies against human pancreatic exocrine cancer: a study of six different immunisation regimes.
title_sort generation of monoclonal antibodies against human pancreatic exocrine cancer: a study of six different immunisation regimes.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977238/
https://www.ncbi.nlm.nih.gov/pubmed/4063133
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