Cloning of human lung cancer cells.

We have carried out a comparison of two different methods for cloning human lung cancer cells. The method of Courtenay & Mills (1978) generally gave higher plating efficiencies (PE) than the method of Carney et al. (1980). The number of colonies increased with incubation time in both methods and...

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Detalles Bibliográficos
Autores principales: Walls, G. A., Twentyman, P. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1985
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977242/
https://www.ncbi.nlm.nih.gov/pubmed/3904799
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author Walls, G. A.
Twentyman, P. R.
author_facet Walls, G. A.
Twentyman, P. R.
author_sort Walls, G. A.
collection PubMed
description We have carried out a comparison of two different methods for cloning human lung cancer cells. The method of Courtenay & Mills (1978) generally gave higher plating efficiencies (PE) than the method of Carney et al. (1980). The number of colonies increased with incubation time in both methods and the weekly medium replenishment in the Courtenay method was advantageous for longer incubation times of several weeks. In the Courtenay method, the use of August rat red blood cells (RBC) and low oxygen tension were both found to be necessary factors for maximum plating efficiency. The usefulness of heavily irradiated feeder cells in improving PE is less certain; each cell type may have its own requirement.
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spelling pubmed-19772422009-09-10 Cloning of human lung cancer cells. Walls, G. A. Twentyman, P. R. Br J Cancer Research Article We have carried out a comparison of two different methods for cloning human lung cancer cells. The method of Courtenay & Mills (1978) generally gave higher plating efficiencies (PE) than the method of Carney et al. (1980). The number of colonies increased with incubation time in both methods and the weekly medium replenishment in the Courtenay method was advantageous for longer incubation times of several weeks. In the Courtenay method, the use of August rat red blood cells (RBC) and low oxygen tension were both found to be necessary factors for maximum plating efficiency. The usefulness of heavily irradiated feeder cells in improving PE is less certain; each cell type may have its own requirement. Nature Publishing Group 1985-10 /pmc/articles/PMC1977242/ /pubmed/3904799 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Walls, G. A.
Twentyman, P. R.
Cloning of human lung cancer cells.
title Cloning of human lung cancer cells.
title_full Cloning of human lung cancer cells.
title_fullStr Cloning of human lung cancer cells.
title_full_unstemmed Cloning of human lung cancer cells.
title_short Cloning of human lung cancer cells.
title_sort cloning of human lung cancer cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977242/
https://www.ncbi.nlm.nih.gov/pubmed/3904799
work_keys_str_mv AT wallsga cloningofhumanlungcancercells
AT twentymanpr cloningofhumanlungcancercells

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