The identification of heme oxygenase as a major hypoxic stress protein in Chinese hamster ovary cells.

Chronic hypoxia increases the expression of a set of stress proteins (oxygen regulated proteins or ORPs) which is implicated in the development of drug resistance and radiation sensitivity in tumour cells. Five major ORPs have been documented, and two, ORP 80 and ORP 100, are considered to be identi...

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Autores principales: Murphy, B. J., Laderoute, K. R., Short, S. M., Sutherland, R. M.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1991
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977299/
https://www.ncbi.nlm.nih.gov/pubmed/1854629
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author Murphy, B. J.
Laderoute, K. R.
Short, S. M.
Sutherland, R. M.
author_facet Murphy, B. J.
Laderoute, K. R.
Short, S. M.
Sutherland, R. M.
author_sort Murphy, B. J.
collection PubMed
description Chronic hypoxia increases the expression of a set of stress proteins (oxygen regulated proteins or ORPs) which is implicated in the development of drug resistance and radiation sensitivity in tumour cells. Five major ORPs have been documented, and two, ORP 80 and ORP 100, are considered to be identical to the glucose regulated stress proteins GRP78 and GRP94, respectively. We report here that ORP 33 is a form of the heme catabolic enzyme, heme oxygenase, using evidence obtained from northern blotting, two-dimensional polyacrylamide gel electrophoresis and western analysis. Heme oxygenase is believed to be an important component of the cellular response to oxidative stress. The significance of heme oxygenase as a hypoxia-induced stress protein is discussed. IMAGES:
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spelling pubmed-19772992009-09-10 The identification of heme oxygenase as a major hypoxic stress protein in Chinese hamster ovary cells. Murphy, B. J. Laderoute, K. R. Short, S. M. Sutherland, R. M. Br J Cancer Research Article Chronic hypoxia increases the expression of a set of stress proteins (oxygen regulated proteins or ORPs) which is implicated in the development of drug resistance and radiation sensitivity in tumour cells. Five major ORPs have been documented, and two, ORP 80 and ORP 100, are considered to be identical to the glucose regulated stress proteins GRP78 and GRP94, respectively. We report here that ORP 33 is a form of the heme catabolic enzyme, heme oxygenase, using evidence obtained from northern blotting, two-dimensional polyacrylamide gel electrophoresis and western analysis. Heme oxygenase is believed to be an important component of the cellular response to oxidative stress. The significance of heme oxygenase as a hypoxia-induced stress protein is discussed. IMAGES: Nature Publishing Group 1991-07 /pmc/articles/PMC1977299/ /pubmed/1854629 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Murphy, B. J.
Laderoute, K. R.
Short, S. M.
Sutherland, R. M.
The identification of heme oxygenase as a major hypoxic stress protein in Chinese hamster ovary cells.
title The identification of heme oxygenase as a major hypoxic stress protein in Chinese hamster ovary cells.
title_full The identification of heme oxygenase as a major hypoxic stress protein in Chinese hamster ovary cells.
title_fullStr The identification of heme oxygenase as a major hypoxic stress protein in Chinese hamster ovary cells.
title_full_unstemmed The identification of heme oxygenase as a major hypoxic stress protein in Chinese hamster ovary cells.
title_short The identification of heme oxygenase as a major hypoxic stress protein in Chinese hamster ovary cells.
title_sort identification of heme oxygenase as a major hypoxic stress protein in chinese hamster ovary cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977299/
https://www.ncbi.nlm.nih.gov/pubmed/1854629
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