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Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma.
Mouse monoclonal antibodies PAb 240 and PAb 1801 which specifically immunoprecipitate p53 protein, were used to examine 27 fresh ovarian tumours (16 serous adenocarcinomas, six endometrioid carcinomas, one mucinous adenocarcinoma, one mucinous borderline tumour and three benign adenomas). Eleven out...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1992
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977350/ https://www.ncbi.nlm.nih.gov/pubmed/1310251 |
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author | Eccles, D. M. Brett, L. Lessells, A. Gruber, L. Lane, D. Steel, C. M. Leonard, R. C. |
author_facet | Eccles, D. M. Brett, L. Lessells, A. Gruber, L. Lane, D. Steel, C. M. Leonard, R. C. |
author_sort | Eccles, D. M. |
collection | PubMed |
description | Mouse monoclonal antibodies PAb 240 and PAb 1801 which specifically immunoprecipitate p53 protein, were used to examine 27 fresh ovarian tumours (16 serous adenocarcinomas, six endometrioid carcinomas, one mucinous adenocarcinoma, one mucinous borderline tumour and three benign adenomas). Eleven out of 16 (69%) serous adenocarcinomas and one endometrioid tumour showed positive staining with one or both antibodies and none of the mucinous or benign tumours stained with either antibody. DNA from tumour and peripheral blood leukocytes was used to identify allelic deletions on chromosome 17p in tumours. 11/12 positively staining tumours showed less of heterozygosity (LOH) on 17p at the nearest informative locus to the p53 gene. In this series of ovarian tumours, LOH on 17p correlates closely with the aberrant expression of the p53 protein in a high proportion of advanced stage serous adenocarcinomas. This observation suggests that the p53 tumour suppressor gene is involved in the evolution of epithelial ovarian cancer (EOC) and may have prognostic significance. IMAGES: |
format | Text |
id | pubmed-1977350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1992 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-19773502009-09-10 Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma. Eccles, D. M. Brett, L. Lessells, A. Gruber, L. Lane, D. Steel, C. M. Leonard, R. C. Br J Cancer Research Article Mouse monoclonal antibodies PAb 240 and PAb 1801 which specifically immunoprecipitate p53 protein, were used to examine 27 fresh ovarian tumours (16 serous adenocarcinomas, six endometrioid carcinomas, one mucinous adenocarcinoma, one mucinous borderline tumour and three benign adenomas). Eleven out of 16 (69%) serous adenocarcinomas and one endometrioid tumour showed positive staining with one or both antibodies and none of the mucinous or benign tumours stained with either antibody. DNA from tumour and peripheral blood leukocytes was used to identify allelic deletions on chromosome 17p in tumours. 11/12 positively staining tumours showed less of heterozygosity (LOH) on 17p at the nearest informative locus to the p53 gene. In this series of ovarian tumours, LOH on 17p correlates closely with the aberrant expression of the p53 protein in a high proportion of advanced stage serous adenocarcinomas. This observation suggests that the p53 tumour suppressor gene is involved in the evolution of epithelial ovarian cancer (EOC) and may have prognostic significance. IMAGES: Nature Publishing Group 1992-01 /pmc/articles/PMC1977350/ /pubmed/1310251 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Eccles, D. M. Brett, L. Lessells, A. Gruber, L. Lane, D. Steel, C. M. Leonard, R. C. Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma. |
title | Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma. |
title_full | Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma. |
title_fullStr | Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma. |
title_full_unstemmed | Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma. |
title_short | Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma. |
title_sort | overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977350/ https://www.ncbi.nlm.nih.gov/pubmed/1310251 |
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