Induction of HSP70 is associated with vincristine resistance in heat-shocked 9L rat brain tumour cells.

The most prominent cellular changes in heat-shock response are induction of HSPs synthesis and reorganisation of cytoskeleton. Vincristine was used as a tool to evaluate the integrity of microtubules in 9L rat brain tumour cells recovering from heat-shock treatment. Cells treated at 45 degrees C for...

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Autores principales: Lee, W. C., Lin, K. Y., Chen, K. D., Lai, Y. K.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1992
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977439/
https://www.ncbi.nlm.nih.gov/pubmed/1419602
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author Lee, W. C.
Lin, K. Y.
Chen, K. D.
Lai, Y. K.
author_facet Lee, W. C.
Lin, K. Y.
Chen, K. D.
Lai, Y. K.
author_sort Lee, W. C.
collection PubMed
description The most prominent cellular changes in heat-shock response are induction of HSPs synthesis and reorganisation of cytoskeleton. Vincristine was used as a tool to evaluate the integrity of microtubules in 9L rat brain tumour cells recovering from heat-shock treatment. Cells treated at 45 degrees C for 15 min and recovered under normal growing condition became resistant to vincristine-inflicted cytotoxicity and microtubule destruction. Among all HSPs, the level of HSP70 and the degree of vincristine resistance are best correlated. HSP70 and tubulin were found to be associated with each other as they were co-immunoprecipitated by either anti-HSP70 or anti-beta-tubulin monoclonal antibody. The current studies establish for the first time that HSP70 can complex with tubulin in cells and this association may stabilise the organisation of microtubules thus protect the heat-treated cells from vincristine damage. These findings are noteworthy in combining hyperthermia and chemotherapy in the management of malignant diseases. IMAGES:
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spelling pubmed-19774392009-09-10 Induction of HSP70 is associated with vincristine resistance in heat-shocked 9L rat brain tumour cells. Lee, W. C. Lin, K. Y. Chen, K. D. Lai, Y. K. Br J Cancer Research Article The most prominent cellular changes in heat-shock response are induction of HSPs synthesis and reorganisation of cytoskeleton. Vincristine was used as a tool to evaluate the integrity of microtubules in 9L rat brain tumour cells recovering from heat-shock treatment. Cells treated at 45 degrees C for 15 min and recovered under normal growing condition became resistant to vincristine-inflicted cytotoxicity and microtubule destruction. Among all HSPs, the level of HSP70 and the degree of vincristine resistance are best correlated. HSP70 and tubulin were found to be associated with each other as they were co-immunoprecipitated by either anti-HSP70 or anti-beta-tubulin monoclonal antibody. The current studies establish for the first time that HSP70 can complex with tubulin in cells and this association may stabilise the organisation of microtubules thus protect the heat-treated cells from vincristine damage. These findings are noteworthy in combining hyperthermia and chemotherapy in the management of malignant diseases. IMAGES: Nature Publishing Group 1992-10 /pmc/articles/PMC1977439/ /pubmed/1419602 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Lee, W. C.
Lin, K. Y.
Chen, K. D.
Lai, Y. K.
Induction of HSP70 is associated with vincristine resistance in heat-shocked 9L rat brain tumour cells.
title Induction of HSP70 is associated with vincristine resistance in heat-shocked 9L rat brain tumour cells.
title_full Induction of HSP70 is associated with vincristine resistance in heat-shocked 9L rat brain tumour cells.
title_fullStr Induction of HSP70 is associated with vincristine resistance in heat-shocked 9L rat brain tumour cells.
title_full_unstemmed Induction of HSP70 is associated with vincristine resistance in heat-shocked 9L rat brain tumour cells.
title_short Induction of HSP70 is associated with vincristine resistance in heat-shocked 9L rat brain tumour cells.
title_sort induction of hsp70 is associated with vincristine resistance in heat-shocked 9l rat brain tumour cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977439/
https://www.ncbi.nlm.nih.gov/pubmed/1419602
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