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Immunohistochemical characterisation of extracellular matrix components of salivary gland tumours.

Proteoglycans (PGs) were localised immunohistochemically in 52 salivary gland tumours including pleomorphic adenoma, adenoid cystic carcinoma, acinic cell carcinoma, oncocytoma, mucoepidermoid carcinoma, clear cell tumour and Warthin tumour, using antibodies raised against large PG, small PG, chondr...

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Autores principales: Nara, Y., Takeuchi, J., Yoshida, K., Fukatsu, T., Nagasaka, T., Kawaguchi, T., Meng, N., Kikuchi, H., Nakashima, N.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1991
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977529/
https://www.ncbi.nlm.nih.gov/pubmed/1909886
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author Nara, Y.
Takeuchi, J.
Yoshida, K.
Fukatsu, T.
Nagasaka, T.
Kawaguchi, T.
Meng, N.
Kikuchi, H.
Nakashima, N.
author_facet Nara, Y.
Takeuchi, J.
Yoshida, K.
Fukatsu, T.
Nagasaka, T.
Kawaguchi, T.
Meng, N.
Kikuchi, H.
Nakashima, N.
author_sort Nara, Y.
collection PubMed
description Proteoglycans (PGs) were localised immunohistochemically in 52 salivary gland tumours including pleomorphic adenoma, adenoid cystic carcinoma, acinic cell carcinoma, oncocytoma, mucoepidermoid carcinoma, clear cell tumour and Warthin tumour, using antibodies raised against large PG, small PG, chondroitin 4-sulphate PG, chondroitin 6-sulphate PG, heparan sulphate PG and keratan sulphate PG. Large PGs were mainly observed in mucinous materials of extracellular matrix (ECM) and interstitial fibrous element of tumour tissues, while small PGs were located only in hyaline matrix and surrounding fibrous (capsular) connective tissues. Chondroitin 6-sulphate PG was detected in the ECM of pleomorphic adenomas and clear cell carcinomas and in pseudocystic spaces of adenoid cystic carcinomas, but only in vessel walls in non-neoplastic tissues. Keratan sulphate PG was observed to locate in mucinous material of pleomorphic adenomas, acinic cell carcinomas and clear cell carcinomas, but not in the adenoid cystic carcinomas examined, and it was also unobservable in non-neoplastic salivary gland tissues. Heparan sulphate PG was observed on the inner surfaces of true ductal spaces of adenoid cystic carcinomas and on cell surfaces of oncocytoma cells. By HPLC analysis, individual glycosaminoglycans contained in tumour tissues were compared. Chondroitin 6-sulphate PG was very rich in ECM of pleomorphic adenomas and adenoid cystic carcinomas. Pleomorphic adenomas contained relatively more low-sulphated chondroitin sulphate than adenoid cystic carcinomas and other tumours. IMAGES:
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spelling pubmed-19775292009-09-10 Immunohistochemical characterisation of extracellular matrix components of salivary gland tumours. Nara, Y. Takeuchi, J. Yoshida, K. Fukatsu, T. Nagasaka, T. Kawaguchi, T. Meng, N. Kikuchi, H. Nakashima, N. Br J Cancer Research Article Proteoglycans (PGs) were localised immunohistochemically in 52 salivary gland tumours including pleomorphic adenoma, adenoid cystic carcinoma, acinic cell carcinoma, oncocytoma, mucoepidermoid carcinoma, clear cell tumour and Warthin tumour, using antibodies raised against large PG, small PG, chondroitin 4-sulphate PG, chondroitin 6-sulphate PG, heparan sulphate PG and keratan sulphate PG. Large PGs were mainly observed in mucinous materials of extracellular matrix (ECM) and interstitial fibrous element of tumour tissues, while small PGs were located only in hyaline matrix and surrounding fibrous (capsular) connective tissues. Chondroitin 6-sulphate PG was detected in the ECM of pleomorphic adenomas and clear cell carcinomas and in pseudocystic spaces of adenoid cystic carcinomas, but only in vessel walls in non-neoplastic tissues. Keratan sulphate PG was observed to locate in mucinous material of pleomorphic adenomas, acinic cell carcinomas and clear cell carcinomas, but not in the adenoid cystic carcinomas examined, and it was also unobservable in non-neoplastic salivary gland tissues. Heparan sulphate PG was observed on the inner surfaces of true ductal spaces of adenoid cystic carcinomas and on cell surfaces of oncocytoma cells. By HPLC analysis, individual glycosaminoglycans contained in tumour tissues were compared. Chondroitin 6-sulphate PG was very rich in ECM of pleomorphic adenomas and adenoid cystic carcinomas. Pleomorphic adenomas contained relatively more low-sulphated chondroitin sulphate than adenoid cystic carcinomas and other tumours. IMAGES: Nature Publishing Group 1991-08 /pmc/articles/PMC1977529/ /pubmed/1909886 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Nara, Y.
Takeuchi, J.
Yoshida, K.
Fukatsu, T.
Nagasaka, T.
Kawaguchi, T.
Meng, N.
Kikuchi, H.
Nakashima, N.
Immunohistochemical characterisation of extracellular matrix components of salivary gland tumours.
title Immunohistochemical characterisation of extracellular matrix components of salivary gland tumours.
title_full Immunohistochemical characterisation of extracellular matrix components of salivary gland tumours.
title_fullStr Immunohistochemical characterisation of extracellular matrix components of salivary gland tumours.
title_full_unstemmed Immunohistochemical characterisation of extracellular matrix components of salivary gland tumours.
title_short Immunohistochemical characterisation of extracellular matrix components of salivary gland tumours.
title_sort immunohistochemical characterisation of extracellular matrix components of salivary gland tumours.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977529/
https://www.ncbi.nlm.nih.gov/pubmed/1909886
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