Cargando…

Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer.

We quantified and examined the kinetics of DNA interstrand cross links (DNA-ISC) caused by Cis dichlorodiammine platinum (DDP) using the method of alkaline elution in 58 highly purified human ovarian tumours growing in primary culture. A large heterogeneity in both the quantity and kinetics of DDP i...

Descripción completa

Detalles Bibliográficos
Autores principales: Balconi, G., Pang, Y., Broggini, M., Morali, F., Marzola, M., Erba, E., Ponti, M., Spinelli, L., Mangioni, C., Redaelli, L.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1991
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977530/
https://www.ncbi.nlm.nih.gov/pubmed/1892757
_version_ 1782135281078501376
author Balconi, G.
Pang, Y.
Broggini, M.
Morali, F.
Marzola, M.
Erba, E.
Ponti, M.
Spinelli, L.
Mangioni, C.
Redaelli, L.
author_facet Balconi, G.
Pang, Y.
Broggini, M.
Morali, F.
Marzola, M.
Erba, E.
Ponti, M.
Spinelli, L.
Mangioni, C.
Redaelli, L.
author_sort Balconi, G.
collection PubMed
description We quantified and examined the kinetics of DNA interstrand cross links (DNA-ISC) caused by Cis dichlorodiammine platinum (DDP) using the method of alkaline elution in 58 highly purified human ovarian tumours growing in primary culture. A large heterogeneity in both the quantity and kinetics of DDP induced DNA-ISC was observed in cultures derived from neoplasms of different patients and from different lesions of the same patient. In the majority of cases. DNA-ISC lasted for prolonged time intervals after 1 h drug exposure, being significantly repaired only 48 or 72 h following drug washout. The persistence of DNA-ISC is probably due to a prolonged formation of these lesions for up to 24 h as assessed by the change in the repair kinetics that occurred after preventing new DNA-ISC formation by quenching of monoadducts with thiourea. The inefficient repair of DDP monoadducts appears therefore to be a possible reason for the permanence of DNA-ISC. These studies suggest that the long permanence of DNA-ISC in human ovarian cancer could be the basis for the high selectivity of DDP for this human malignancy.
format Text
id pubmed-1977530
institution National Center for Biotechnology Information
language English
publishDate 1991
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-19775302009-09-10 Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer. Balconi, G. Pang, Y. Broggini, M. Morali, F. Marzola, M. Erba, E. Ponti, M. Spinelli, L. Mangioni, C. Redaelli, L. Br J Cancer Research Article We quantified and examined the kinetics of DNA interstrand cross links (DNA-ISC) caused by Cis dichlorodiammine platinum (DDP) using the method of alkaline elution in 58 highly purified human ovarian tumours growing in primary culture. A large heterogeneity in both the quantity and kinetics of DDP induced DNA-ISC was observed in cultures derived from neoplasms of different patients and from different lesions of the same patient. In the majority of cases. DNA-ISC lasted for prolonged time intervals after 1 h drug exposure, being significantly repaired only 48 or 72 h following drug washout. The persistence of DNA-ISC is probably due to a prolonged formation of these lesions for up to 24 h as assessed by the change in the repair kinetics that occurred after preventing new DNA-ISC formation by quenching of monoadducts with thiourea. The inefficient repair of DDP monoadducts appears therefore to be a possible reason for the permanence of DNA-ISC. These studies suggest that the long permanence of DNA-ISC in human ovarian cancer could be the basis for the high selectivity of DDP for this human malignancy. Nature Publishing Group 1991-08 /pmc/articles/PMC1977530/ /pubmed/1892757 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Balconi, G.
Pang, Y.
Broggini, M.
Morali, F.
Marzola, M.
Erba, E.
Ponti, M.
Spinelli, L.
Mangioni, C.
Redaelli, L.
Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer.
title Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer.
title_full Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer.
title_fullStr Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer.
title_full_unstemmed Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer.
title_short Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer.
title_sort cis dichlorodiammine platinum induced dna interstrand cross-links in primary cultures of human ovarian cancer.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977530/
https://www.ncbi.nlm.nih.gov/pubmed/1892757
work_keys_str_mv AT balconig cisdichlorodiammineplatinuminduceddnainterstrandcrosslinksinprimaryculturesofhumanovariancancer
AT pangy cisdichlorodiammineplatinuminduceddnainterstrandcrosslinksinprimaryculturesofhumanovariancancer
AT brogginim cisdichlorodiammineplatinuminduceddnainterstrandcrosslinksinprimaryculturesofhumanovariancancer
AT moralif cisdichlorodiammineplatinuminduceddnainterstrandcrosslinksinprimaryculturesofhumanovariancancer
AT marzolam cisdichlorodiammineplatinuminduceddnainterstrandcrosslinksinprimaryculturesofhumanovariancancer
AT erbae cisdichlorodiammineplatinuminduceddnainterstrandcrosslinksinprimaryculturesofhumanovariancancer
AT pontim cisdichlorodiammineplatinuminduceddnainterstrandcrosslinksinprimaryculturesofhumanovariancancer
AT spinellil cisdichlorodiammineplatinuminduceddnainterstrandcrosslinksinprimaryculturesofhumanovariancancer
AT mangionic cisdichlorodiammineplatinuminduceddnainterstrandcrosslinksinprimaryculturesofhumanovariancancer
AT redaellil cisdichlorodiammineplatinuminduceddnainterstrandcrosslinksinprimaryculturesofhumanovariancancer