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Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer.
We quantified and examined the kinetics of DNA interstrand cross links (DNA-ISC) caused by Cis dichlorodiammine platinum (DDP) using the method of alkaline elution in 58 highly purified human ovarian tumours growing in primary culture. A large heterogeneity in both the quantity and kinetics of DDP i...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1991
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977530/ https://www.ncbi.nlm.nih.gov/pubmed/1892757 |
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author | Balconi, G. Pang, Y. Broggini, M. Morali, F. Marzola, M. Erba, E. Ponti, M. Spinelli, L. Mangioni, C. Redaelli, L. |
author_facet | Balconi, G. Pang, Y. Broggini, M. Morali, F. Marzola, M. Erba, E. Ponti, M. Spinelli, L. Mangioni, C. Redaelli, L. |
author_sort | Balconi, G. |
collection | PubMed |
description | We quantified and examined the kinetics of DNA interstrand cross links (DNA-ISC) caused by Cis dichlorodiammine platinum (DDP) using the method of alkaline elution in 58 highly purified human ovarian tumours growing in primary culture. A large heterogeneity in both the quantity and kinetics of DDP induced DNA-ISC was observed in cultures derived from neoplasms of different patients and from different lesions of the same patient. In the majority of cases. DNA-ISC lasted for prolonged time intervals after 1 h drug exposure, being significantly repaired only 48 or 72 h following drug washout. The persistence of DNA-ISC is probably due to a prolonged formation of these lesions for up to 24 h as assessed by the change in the repair kinetics that occurred after preventing new DNA-ISC formation by quenching of monoadducts with thiourea. The inefficient repair of DDP monoadducts appears therefore to be a possible reason for the permanence of DNA-ISC. These studies suggest that the long permanence of DNA-ISC in human ovarian cancer could be the basis for the high selectivity of DDP for this human malignancy. |
format | Text |
id | pubmed-1977530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1991 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-19775302009-09-10 Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer. Balconi, G. Pang, Y. Broggini, M. Morali, F. Marzola, M. Erba, E. Ponti, M. Spinelli, L. Mangioni, C. Redaelli, L. Br J Cancer Research Article We quantified and examined the kinetics of DNA interstrand cross links (DNA-ISC) caused by Cis dichlorodiammine platinum (DDP) using the method of alkaline elution in 58 highly purified human ovarian tumours growing in primary culture. A large heterogeneity in both the quantity and kinetics of DDP induced DNA-ISC was observed in cultures derived from neoplasms of different patients and from different lesions of the same patient. In the majority of cases. DNA-ISC lasted for prolonged time intervals after 1 h drug exposure, being significantly repaired only 48 or 72 h following drug washout. The persistence of DNA-ISC is probably due to a prolonged formation of these lesions for up to 24 h as assessed by the change in the repair kinetics that occurred after preventing new DNA-ISC formation by quenching of monoadducts with thiourea. The inefficient repair of DDP monoadducts appears therefore to be a possible reason for the permanence of DNA-ISC. These studies suggest that the long permanence of DNA-ISC in human ovarian cancer could be the basis for the high selectivity of DDP for this human malignancy. Nature Publishing Group 1991-08 /pmc/articles/PMC1977530/ /pubmed/1892757 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Balconi, G. Pang, Y. Broggini, M. Morali, F. Marzola, M. Erba, E. Ponti, M. Spinelli, L. Mangioni, C. Redaelli, L. Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer. |
title | Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer. |
title_full | Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer. |
title_fullStr | Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer. |
title_full_unstemmed | Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer. |
title_short | Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer. |
title_sort | cis dichlorodiammine platinum induced dna interstrand cross-links in primary cultures of human ovarian cancer. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977530/ https://www.ncbi.nlm.nih.gov/pubmed/1892757 |
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