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IL-1 production as a regulator of G-CSF and IL-6 production in CSF-producing cell lines.
We previously demonstrated that colony stimulating factor (CSF)-producing cell lines co-produce interleukin-1 (IL-1) and IL-6 in addition to CSFs. In the present study, we examined the role of IL-1 production in three human tumour cell lines producing granulocyte (G)-CSF, IL-1 and IL-6. Addition of...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1992
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977563/ https://www.ncbi.nlm.nih.gov/pubmed/1373292 |
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author | Suzuki, A. Takahashi, T. Okuno, Y. Tsuyuoka, R. Fukumoto, M. Nakamura, K. Imura, H. |
author_facet | Suzuki, A. Takahashi, T. Okuno, Y. Tsuyuoka, R. Fukumoto, M. Nakamura, K. Imura, H. |
author_sort | Suzuki, A. |
collection | PubMed |
description | We previously demonstrated that colony stimulating factor (CSF)-producing cell lines co-produce interleukin-1 (IL-1) and IL-6 in addition to CSFs. In the present study, we examined the role of IL-1 production in three human tumour cell lines producing granulocyte (G)-CSF, IL-1 and IL-6. Addition of anti-human IL-1 alpha antiserum to the culture caused a 90-62% reduction of G-CSF and a 85-44% reduction of IL-6 production, respectively, as evaluated by enzyme immunoassay in all three cell lines. The decrease of G-CSF and IL-6 production by the anti-IL-1 alpha antiserum was also confirmed at the level of mRNA expression. The anti-IL-1 alpha antiserum did not affect the growth of these cell lines. Excess recombinant IL-1 alpha exogenously added to the culture enhanced G-CSF and IL-6 production in all three cell lines. However, IL-1 alpha had little effect on the growth of these three cell lines. Neither anti-IL-6 nor anti-G-CSF antibodies affected the production of the other cytokines. These results indicate that IL-1 alpha regulates G-CSF and IL-6 production in these tumour cell lines, and suggest that the IL-1 production plays an important role in CSF-producing tumours. IMAGES: |
format | Text |
id | pubmed-1977563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1992 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-19775632009-09-10 IL-1 production as a regulator of G-CSF and IL-6 production in CSF-producing cell lines. Suzuki, A. Takahashi, T. Okuno, Y. Tsuyuoka, R. Fukumoto, M. Nakamura, K. Imura, H. Br J Cancer Research Article We previously demonstrated that colony stimulating factor (CSF)-producing cell lines co-produce interleukin-1 (IL-1) and IL-6 in addition to CSFs. In the present study, we examined the role of IL-1 production in three human tumour cell lines producing granulocyte (G)-CSF, IL-1 and IL-6. Addition of anti-human IL-1 alpha antiserum to the culture caused a 90-62% reduction of G-CSF and a 85-44% reduction of IL-6 production, respectively, as evaluated by enzyme immunoassay in all three cell lines. The decrease of G-CSF and IL-6 production by the anti-IL-1 alpha antiserum was also confirmed at the level of mRNA expression. The anti-IL-1 alpha antiserum did not affect the growth of these cell lines. Excess recombinant IL-1 alpha exogenously added to the culture enhanced G-CSF and IL-6 production in all three cell lines. However, IL-1 alpha had little effect on the growth of these three cell lines. Neither anti-IL-6 nor anti-G-CSF antibodies affected the production of the other cytokines. These results indicate that IL-1 alpha regulates G-CSF and IL-6 production in these tumour cell lines, and suggest that the IL-1 production plays an important role in CSF-producing tumours. IMAGES: Nature Publishing Group 1992-04 /pmc/articles/PMC1977563/ /pubmed/1373292 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Suzuki, A. Takahashi, T. Okuno, Y. Tsuyuoka, R. Fukumoto, M. Nakamura, K. Imura, H. IL-1 production as a regulator of G-CSF and IL-6 production in CSF-producing cell lines. |
title | IL-1 production as a regulator of G-CSF and IL-6 production in CSF-producing cell lines. |
title_full | IL-1 production as a regulator of G-CSF and IL-6 production in CSF-producing cell lines. |
title_fullStr | IL-1 production as a regulator of G-CSF and IL-6 production in CSF-producing cell lines. |
title_full_unstemmed | IL-1 production as a regulator of G-CSF and IL-6 production in CSF-producing cell lines. |
title_short | IL-1 production as a regulator of G-CSF and IL-6 production in CSF-producing cell lines. |
title_sort | il-1 production as a regulator of g-csf and il-6 production in csf-producing cell lines. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977563/ https://www.ncbi.nlm.nih.gov/pubmed/1373292 |
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