Transcriptional down-regulation of c-myc in human prostate carcinoma cells by the synthetic androgen mibolerone.

The mechanism of down-regulation of c-myc RNA associated with androgen-induced suppression of the transformed phenotype in the human prostate carcinoma cell line LNCaP was investigated. The synthetic androgen mibolerone (7 alpha-17 alpha-Dimethyl-19-nortestosterone) reversibly inhibits the prolifera...

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Detalles Bibliográficos
Autores principales: Wolf, D. A., Kohlhuber, F., Schulz, P., Fittler, F., Eick, D.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1992
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977595/
https://www.ncbi.nlm.nih.gov/pubmed/1373070
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author Wolf, D. A.
Kohlhuber, F.
Schulz, P.
Fittler, F.
Eick, D.
author_facet Wolf, D. A.
Kohlhuber, F.
Schulz, P.
Fittler, F.
Eick, D.
author_sort Wolf, D. A.
collection PubMed
description The mechanism of down-regulation of c-myc RNA associated with androgen-induced suppression of the transformed phenotype in the human prostate carcinoma cell line LNCaP was investigated. The synthetic androgen mibolerone (7 alpha-17 alpha-Dimethyl-19-nortestosterone) reversibly inhibits the proliferation of LNCaP cells and, from 12-72 h after hormone addition reduces the level of c-myc transcripts to a few per cent of controls. P1, P2, and P0 c-myc transcripts decline at the same rate, whereas P3 transcripts are much less hormone sensitive. Nuclear run-on analysis revealed that c-myc is down-regulated at the level of transcription initiation in LNCaP cells. The level of c-myc transcripts prevailing in untreated control cells can be restored in androgen-induced cells by excess antiandrogen, indicating the involvement of the androgen receptor in c-myc down-regulation. IMAGES:
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spelling pubmed-19775952009-09-10 Transcriptional down-regulation of c-myc in human prostate carcinoma cells by the synthetic androgen mibolerone. Wolf, D. A. Kohlhuber, F. Schulz, P. Fittler, F. Eick, D. Br J Cancer Research Article The mechanism of down-regulation of c-myc RNA associated with androgen-induced suppression of the transformed phenotype in the human prostate carcinoma cell line LNCaP was investigated. The synthetic androgen mibolerone (7 alpha-17 alpha-Dimethyl-19-nortestosterone) reversibly inhibits the proliferation of LNCaP cells and, from 12-72 h after hormone addition reduces the level of c-myc transcripts to a few per cent of controls. P1, P2, and P0 c-myc transcripts decline at the same rate, whereas P3 transcripts are much less hormone sensitive. Nuclear run-on analysis revealed that c-myc is down-regulated at the level of transcription initiation in LNCaP cells. The level of c-myc transcripts prevailing in untreated control cells can be restored in androgen-induced cells by excess antiandrogen, indicating the involvement of the androgen receptor in c-myc down-regulation. IMAGES: Nature Publishing Group 1992-03 /pmc/articles/PMC1977595/ /pubmed/1373070 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Wolf, D. A.
Kohlhuber, F.
Schulz, P.
Fittler, F.
Eick, D.
Transcriptional down-regulation of c-myc in human prostate carcinoma cells by the synthetic androgen mibolerone.
title Transcriptional down-regulation of c-myc in human prostate carcinoma cells by the synthetic androgen mibolerone.
title_full Transcriptional down-regulation of c-myc in human prostate carcinoma cells by the synthetic androgen mibolerone.
title_fullStr Transcriptional down-regulation of c-myc in human prostate carcinoma cells by the synthetic androgen mibolerone.
title_full_unstemmed Transcriptional down-regulation of c-myc in human prostate carcinoma cells by the synthetic androgen mibolerone.
title_short Transcriptional down-regulation of c-myc in human prostate carcinoma cells by the synthetic androgen mibolerone.
title_sort transcriptional down-regulation of c-myc in human prostate carcinoma cells by the synthetic androgen mibolerone.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977595/
https://www.ncbi.nlm.nih.gov/pubmed/1373070
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