Establishment and characterisation of human carcinoembryonic antigen transgenic mice.

We have produced human CEA transgenic mice which were found to express CEA mRNA in all tissues. By immunoblot analysis using anti-CEA polyclonal antibody, we also detected CEA protein in all tissues. However, the molecular size of CEA in the brain was different from that in other tissues, although t...

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Autores principales: Hasegawa, T., Isobe, K., Tsuchiya, Y., Oikawa, S., Nakazato, H., Ikezawa, H., Nakashima, I., Shimokata, K.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1991
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977681/
https://www.ncbi.nlm.nih.gov/pubmed/1911219
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author Hasegawa, T.
Isobe, K.
Tsuchiya, Y.
Oikawa, S.
Nakazato, H.
Ikezawa, H.
Nakashima, I.
Shimokata, K.
author_facet Hasegawa, T.
Isobe, K.
Tsuchiya, Y.
Oikawa, S.
Nakazato, H.
Ikezawa, H.
Nakashima, I.
Shimokata, K.
author_sort Hasegawa, T.
collection PubMed
description We have produced human CEA transgenic mice which were found to express CEA mRNA in all tissues. By immunoblot analysis using anti-CEA polyclonal antibody, we also detected CEA protein in all tissues. However, the molecular size of CEA in the brain was different from that in other tissues, although the mRNA size was same and no deletion nor rearrangement was detected at the DNA level. Immunohistochemical analysis of the lung and the colon showed that the expression sites were the bronchial epithelial cells of the lung and the columnar epithelial cells of the colon. Interestingly, the expression of CEA protein in the transgenic mice was polarised to the luminal side of epithelial cells similar to the normal CEA expression in human tissues. We also detected cell surface expression of human CEA on thymocytes and spleen cells and CEA expression was greatly reduced by the phosphatidylinositol-specific phospholipase C (PI-PLC) treatment. IMAGES:
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spelling pubmed-19776812009-09-10 Establishment and characterisation of human carcinoembryonic antigen transgenic mice. Hasegawa, T. Isobe, K. Tsuchiya, Y. Oikawa, S. Nakazato, H. Ikezawa, H. Nakashima, I. Shimokata, K. Br J Cancer Research Article We have produced human CEA transgenic mice which were found to express CEA mRNA in all tissues. By immunoblot analysis using anti-CEA polyclonal antibody, we also detected CEA protein in all tissues. However, the molecular size of CEA in the brain was different from that in other tissues, although the mRNA size was same and no deletion nor rearrangement was detected at the DNA level. Immunohistochemical analysis of the lung and the colon showed that the expression sites were the bronchial epithelial cells of the lung and the columnar epithelial cells of the colon. Interestingly, the expression of CEA protein in the transgenic mice was polarised to the luminal side of epithelial cells similar to the normal CEA expression in human tissues. We also detected cell surface expression of human CEA on thymocytes and spleen cells and CEA expression was greatly reduced by the phosphatidylinositol-specific phospholipase C (PI-PLC) treatment. IMAGES: Nature Publishing Group 1991-10 /pmc/articles/PMC1977681/ /pubmed/1911219 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Hasegawa, T.
Isobe, K.
Tsuchiya, Y.
Oikawa, S.
Nakazato, H.
Ikezawa, H.
Nakashima, I.
Shimokata, K.
Establishment and characterisation of human carcinoembryonic antigen transgenic mice.
title Establishment and characterisation of human carcinoembryonic antigen transgenic mice.
title_full Establishment and characterisation of human carcinoembryonic antigen transgenic mice.
title_fullStr Establishment and characterisation of human carcinoembryonic antigen transgenic mice.
title_full_unstemmed Establishment and characterisation of human carcinoembryonic antigen transgenic mice.
title_short Establishment and characterisation of human carcinoembryonic antigen transgenic mice.
title_sort establishment and characterisation of human carcinoembryonic antigen transgenic mice.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977681/
https://www.ncbi.nlm.nih.gov/pubmed/1911219
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