XH1--a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression.

A new cell line, XH1, has been derived from an invasive focally keratinising adenosquamous carcinoma of the cervix in a 32 year old patient. It has been maintained in long term monolayer culture for 26 months, and passaged over 100 times (much greater than 300 population doublings). It is aneuploid...

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Autores principales: Han, X., Lyle, R., Eustace, D. L., Jewers, R. J., Parrington, J. M., Das, A., Chana, T., Dagg, B., Money, S., Bates, T. D.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1991
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977698/
https://www.ncbi.nlm.nih.gov/pubmed/1911212
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author Han, X.
Lyle, R.
Eustace, D. L.
Jewers, R. J.
Parrington, J. M.
Das, A.
Chana, T.
Dagg, B.
Money, S.
Bates, T. D.
author_facet Han, X.
Lyle, R.
Eustace, D. L.
Jewers, R. J.
Parrington, J. M.
Das, A.
Chana, T.
Dagg, B.
Money, S.
Bates, T. D.
author_sort Han, X.
collection PubMed
description A new cell line, XH1, has been derived from an invasive focally keratinising adenosquamous carcinoma of the cervix in a 32 year old patient. It has been maintained in long term monolayer culture for 26 months, and passaged over 100 times (much greater than 300 population doublings). It is aneuploid with a mean chromosome number of 78. Examination using two minisatellite hypervariable DNA probes has shown it to be different from other cell lines maintained in this laboratory and from HeLa. Two sublines, XH1a and XH1b, show marked differences in monolayer culture, growth in soft agar, and xenograft formation. XH1 and XH1a cells readily form subcutaneous xenografts, and lung colonies can be established by their intravenous injection. Subcutaneous injection of XH1b cells results in rapid cell growth for a few days after which the tumour undergoes degeneration and then regresses completely. The XH1 karyotype has many rearranged chromosomes. Parental XH1 cells and both sublines show integration of HPV16 into the genome. IMAGES:
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spelling pubmed-19776982009-09-10 XH1--a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression. Han, X. Lyle, R. Eustace, D. L. Jewers, R. J. Parrington, J. M. Das, A. Chana, T. Dagg, B. Money, S. Bates, T. D. Br J Cancer Research Article A new cell line, XH1, has been derived from an invasive focally keratinising adenosquamous carcinoma of the cervix in a 32 year old patient. It has been maintained in long term monolayer culture for 26 months, and passaged over 100 times (much greater than 300 population doublings). It is aneuploid with a mean chromosome number of 78. Examination using two minisatellite hypervariable DNA probes has shown it to be different from other cell lines maintained in this laboratory and from HeLa. Two sublines, XH1a and XH1b, show marked differences in monolayer culture, growth in soft agar, and xenograft formation. XH1 and XH1a cells readily form subcutaneous xenografts, and lung colonies can be established by their intravenous injection. Subcutaneous injection of XH1b cells results in rapid cell growth for a few days after which the tumour undergoes degeneration and then regresses completely. The XH1 karyotype has many rearranged chromosomes. Parental XH1 cells and both sublines show integration of HPV16 into the genome. IMAGES: Nature Publishing Group 1991-10 /pmc/articles/PMC1977698/ /pubmed/1911212 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Han, X.
Lyle, R.
Eustace, D. L.
Jewers, R. J.
Parrington, J. M.
Das, A.
Chana, T.
Dagg, B.
Money, S.
Bates, T. D.
XH1--a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression.
title XH1--a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression.
title_full XH1--a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression.
title_fullStr XH1--a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression.
title_full_unstemmed XH1--a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression.
title_short XH1--a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression.
title_sort xh1--a new cervical carcinoma cell line and xenograft model of tumour invasion, 'metastasis' and regression.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977698/
https://www.ncbi.nlm.nih.gov/pubmed/1911212
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