Cargando…

Divergent responses to epidermal growth factor in hormone sensitive and insensitive human prostate cancer cell lines.

The present study was undertaken to compare the relationship between response to exogenous epidermal growth factor (EGF) and the expression of the EGF-receptor (EGF-R) in an androgen sensitive (LNCaP) and insensitive (DU145) prostate cancer cell line. Although both cell lines demonstrated a single E...

Descripción completa

Detalles Bibliográficos
Autores principales: MacDonald, A., Habib, F. K.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1992
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977738/
https://www.ncbi.nlm.nih.gov/pubmed/1739613
_version_ 1782135326595088384
author MacDonald, A.
Habib, F. K.
author_facet MacDonald, A.
Habib, F. K.
author_sort MacDonald, A.
collection PubMed
description The present study was undertaken to compare the relationship between response to exogenous epidermal growth factor (EGF) and the expression of the EGF-receptor (EGF-R) in an androgen sensitive (LNCaP) and insensitive (DU145) prostate cancer cell line. Although both cell lines demonstrated a single EGF-R binding site of similar high affinities (mean dissociation constant (Kd) +/- S.D. for DU145 = 1.0 +/- 0.6 nmol l-1; LNCaP = 2.8 +/- 2.2 nmol l-1) the number of binding sites (RT) for the hormone insensitive DU145 cells (mean +/- S.D. = 2.5 +/- 1.0 x 10(5) sites/cell) and 10-fold greater than that expressed in the androgen responsive LNCaP cell line (mean +/- S.D. = 2.0 +/- 1 x 10(4) sites/cell). Additionally exogenous EGF only minimally affected the growth and DNA synthesis of DU145 cells whereas LNCaP cells showed a significant response which was dose dependent. The autologous production of EGF-like molecules by DU145 cells is believed to reduce the cells needs for exogenous mitogens, thereby rendering the cells autostimulatory. Treatment of LNCaP cells with Mibolerone--a synthetic androgen--did not affect either the expression of the EGF receptor or the proliferative response observed with EGF. Western blot analysis, using monoclonal antibodies directed against the EGF receptor revealed a band of approximately 170 kD with DU145 cell lysates but the LNCaP EGF receptor was not detected using this technique. IMAGES:
format Text
id pubmed-1977738
institution National Center for Biotechnology Information
language English
publishDate 1992
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-19777382009-09-10 Divergent responses to epidermal growth factor in hormone sensitive and insensitive human prostate cancer cell lines. MacDonald, A. Habib, F. K. Br J Cancer Research Article The present study was undertaken to compare the relationship between response to exogenous epidermal growth factor (EGF) and the expression of the EGF-receptor (EGF-R) in an androgen sensitive (LNCaP) and insensitive (DU145) prostate cancer cell line. Although both cell lines demonstrated a single EGF-R binding site of similar high affinities (mean dissociation constant (Kd) +/- S.D. for DU145 = 1.0 +/- 0.6 nmol l-1; LNCaP = 2.8 +/- 2.2 nmol l-1) the number of binding sites (RT) for the hormone insensitive DU145 cells (mean +/- S.D. = 2.5 +/- 1.0 x 10(5) sites/cell) and 10-fold greater than that expressed in the androgen responsive LNCaP cell line (mean +/- S.D. = 2.0 +/- 1 x 10(4) sites/cell). Additionally exogenous EGF only minimally affected the growth and DNA synthesis of DU145 cells whereas LNCaP cells showed a significant response which was dose dependent. The autologous production of EGF-like molecules by DU145 cells is believed to reduce the cells needs for exogenous mitogens, thereby rendering the cells autostimulatory. Treatment of LNCaP cells with Mibolerone--a synthetic androgen--did not affect either the expression of the EGF receptor or the proliferative response observed with EGF. Western blot analysis, using monoclonal antibodies directed against the EGF receptor revealed a band of approximately 170 kD with DU145 cell lysates but the LNCaP EGF receptor was not detected using this technique. IMAGES: Nature Publishing Group 1992-02 /pmc/articles/PMC1977738/ /pubmed/1739613 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
MacDonald, A.
Habib, F. K.
Divergent responses to epidermal growth factor in hormone sensitive and insensitive human prostate cancer cell lines.
title Divergent responses to epidermal growth factor in hormone sensitive and insensitive human prostate cancer cell lines.
title_full Divergent responses to epidermal growth factor in hormone sensitive and insensitive human prostate cancer cell lines.
title_fullStr Divergent responses to epidermal growth factor in hormone sensitive and insensitive human prostate cancer cell lines.
title_full_unstemmed Divergent responses to epidermal growth factor in hormone sensitive and insensitive human prostate cancer cell lines.
title_short Divergent responses to epidermal growth factor in hormone sensitive and insensitive human prostate cancer cell lines.
title_sort divergent responses to epidermal growth factor in hormone sensitive and insensitive human prostate cancer cell lines.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977738/
https://www.ncbi.nlm.nih.gov/pubmed/1739613
work_keys_str_mv AT macdonalda divergentresponsestoepidermalgrowthfactorinhormonesensitiveandinsensitivehumanprostatecancercelllines
AT habibfk divergentresponsestoepidermalgrowthfactorinhormonesensitiveandinsensitivehumanprostatecancercelllines