The inhibition of DNA synthesis in chronic lymphocytic leukaemia cells by chlorambucil in vitro.

The inhibition of 3H-thymidine incorporation into the DNA of mitogen-stimulated lymphocytes from patients with chronic lymphocytic leukaemia by chlorambucil was measured in vitro and the results related to clinical drug resistance. The assay proved to be both sensitive and specific showing a clear s...

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Detalles Bibliográficos
Autores principales: Bentley, D. P., Blackmore, J. A.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1992
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977741/
https://www.ncbi.nlm.nih.gov/pubmed/1739612
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author Bentley, D. P.
Blackmore, J. A.
author_facet Bentley, D. P.
Blackmore, J. A.
author_sort Bentley, D. P.
collection PubMed
description The inhibition of 3H-thymidine incorporation into the DNA of mitogen-stimulated lymphocytes from patients with chronic lymphocytic leukaemia by chlorambucil was measured in vitro and the results related to clinical drug resistance. The assay proved to be both sensitive and specific showing a clear separation of those patients with responsive disease from those with disease resistant to treatment. There was evidence of primary drug resistance in untreated patients. In almost all patients who received treatment this led to increasing resistance to chlorambucil in vitro. The assay is predictive of clinical responsiveness and provides a potential means whereby new therapeutic agents and treatment modifiers may be investigated.
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spelling pubmed-19777412009-09-10 The inhibition of DNA synthesis in chronic lymphocytic leukaemia cells by chlorambucil in vitro. Bentley, D. P. Blackmore, J. A. Br J Cancer Research Article The inhibition of 3H-thymidine incorporation into the DNA of mitogen-stimulated lymphocytes from patients with chronic lymphocytic leukaemia by chlorambucil was measured in vitro and the results related to clinical drug resistance. The assay proved to be both sensitive and specific showing a clear separation of those patients with responsive disease from those with disease resistant to treatment. There was evidence of primary drug resistance in untreated patients. In almost all patients who received treatment this led to increasing resistance to chlorambucil in vitro. The assay is predictive of clinical responsiveness and provides a potential means whereby new therapeutic agents and treatment modifiers may be investigated. Nature Publishing Group 1992-02 /pmc/articles/PMC1977741/ /pubmed/1739612 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Bentley, D. P.
Blackmore, J. A.
The inhibition of DNA synthesis in chronic lymphocytic leukaemia cells by chlorambucil in vitro.
title The inhibition of DNA synthesis in chronic lymphocytic leukaemia cells by chlorambucil in vitro.
title_full The inhibition of DNA synthesis in chronic lymphocytic leukaemia cells by chlorambucil in vitro.
title_fullStr The inhibition of DNA synthesis in chronic lymphocytic leukaemia cells by chlorambucil in vitro.
title_full_unstemmed The inhibition of DNA synthesis in chronic lymphocytic leukaemia cells by chlorambucil in vitro.
title_short The inhibition of DNA synthesis in chronic lymphocytic leukaemia cells by chlorambucil in vitro.
title_sort inhibition of dna synthesis in chronic lymphocytic leukaemia cells by chlorambucil in vitro.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1977741/
https://www.ncbi.nlm.nih.gov/pubmed/1739612
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